A'inurrofiqin Moh, Rahayu Endang Sutriswati, Suroto Dian Anggraini, Utami Tyas, Mayangsari Yunika
Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia.
University Center of Excellence for Research and Application on Integrated Probiotic Industry, Universitas Gadjah Mada, Yogyakarta, Indonesia.
AIMS Microbiol. 2022 Nov 1;8(4):403-421. doi: 10.3934/microbiol.2022028. eCollection 2022.
subsp. Kita-3 is a candidate probiotic from Halloumi cheese produced by Mazaraat Artisan Cheese, Yogyakarta, Indonesia. This study evaluated the safety of consuming a high dose of subsp. Kita-3 in Sprague-Dawley rats for 28 days. Eighteen male rats were randomly divided into three groups, such as the control group, the skim milk group, and the probiotic group. Feed intake and body weight were monitored, and blood samples, organs (kidneys, spleen, and liver), and the colon were dissected. Organ weight, hematological parameters, serum glutamic oxaloacetic transaminase (SGOT), and serum glutamic pyruvic transaminase (SGPT) concentrations, as well as intestinal morphology of the rats, were measured. Microbial analyses were carried out on the digesta, feces, blood, organs, and colon. The results showed that consumption of did not negatively affect general health, organ weight, hematological parameters, SGOT and SGPT activities, or intestinal morphology. The number of in the feces of rats increased significantly, indicating survival of the bacterium in the gastrointestinal tract. The bacteria in the blood, organs, and colon of all groups were identified using repetitive-polymerase chain reaction with the BOXA1R primers and further by 16S rRNA gene sequencing analysis, which revealed that they were not identical to subsp. Kita-3. Thus, this strain did not translocate to the blood or organs of rats. Therefore, subsp. Kita-3 is likely to be safe for human consumption.
亚种Kita - 3是一种候选益生菌，源自印度尼西亚日惹市Mazaraat手工奶酪公司生产的哈洛米奶酪。本研究评估了在斯普拉格 - 道利大鼠中连续28天高剂量食用亚种Kita - 3的安全性。18只雄性大鼠被随机分为三组，即对照组、脱脂牛奶组和益生菌组。监测饲料摄入量和体重，并采集血液样本、解剖器官（肾脏、脾脏和肝脏）以及结肠。测量大鼠的器官重量、血液学参数、血清谷草转氨酶（SGOT）和血清谷丙转氨酶（SGPT）浓度以及肠道形态。对消化物、粪便、血液、器官和结肠进行微生物分析。结果表明，食用[未提及具体食用物]对大鼠的总体健康、器官重量、血液学参数、SGOT和SGPT活性或肠道形态没有负面影响。大鼠粪便中的[未提及具体细菌]数量显著增加，表明该细菌在胃肠道中存活。使用BOXA1R引物通过重复聚合酶链反应并进一步通过16S rRNA基因测序分析鉴定所有组大鼠血液、器官和结肠中的细菌，结果显示它们与亚种Kita - 3不同。因此，该菌株不会转移到大鼠的血液或器官中。所以，亚种Kita - 3可能对人类食用是安全的。
