Deng Liquan, Liu Liming, Fu Tongyu, Li Chunhua, Jin Ningyi, Zhang Heping, Li Chang, Liu Yawen, Zhao Cuiqing
School of Public Health, Jilin University, Changchun 130021, China.
College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin 132101, China.
Microorganisms. 2023 Jun 20;11(6):1620. doi: 10.3390/microorganisms11061620.
This study aims to systematically evaluate the safety of a novel LPJZ-658 explored on whole-genome sequence analysis, safety, and probiotic properties assessment. Whole genome sequencing results demonstrated that LPJZ-658 consists of 3.26 Mbp with a GC content of 44.83%. A total of 3254 putative ORFs were identified. Of note, a putative bile saline hydrolase (BSH) (identity 70.4%) was found in its genome. In addition, the secondary metabolites were analyzed, and one secondary metabolite gene cluster was predicted to consist of 51 genes, which verified its safety and probiotic properties at the genome level. Additionally, LPJZ-658 exhibited non-toxic and non-hemolytic activity and was susceptible to various tested antibiotics, indicating that LPJZ-658 was safe for consumption. Moreover, the probiotic properties tests confirm that LPJZ-658 also exhibits tolerance to acid and bile salts, preferably hydrophobicity and auto-aggregation, and excellent antimicrobial activity against both Gram-positive and Gram-negative gastrointestinal pathogens. In conclusion, this study confirmed the safety and probiotic properties of LPJZ-658, suggesting it can be used as a potential probiotic candidate for human and animal applications.
本研究旨在系统评估一种新型LPJZ - 658在全基因组序列分析、安全性及益生菌特性评估方面的安全性。全基因组测序结果表明，LPJZ - 658由326万个碱基对组成，GC含量为44.83%。共鉴定出3254个推定的开放阅读框。值得注意的是，在其基因组中发现了一个推定的胆盐水解酶（BSH）（一致性为70.4%）。此外，对次生代谢产物进行了分析，预测一个次生代谢产物基因簇由51个基因组成，这在基因组水平上验证了其安全性和益生菌特性。此外，LPJZ - 658表现出无毒和非溶血活性，并且对各种测试抗生素敏感，表明LPJZ - 658食用安全。此外，益生菌特性测试证实，LPJZ - 658还表现出对酸和胆盐的耐受性、较好的疏水性和自聚集性，以及对革兰氏阳性和革兰氏阴性胃肠道病原体的优异抗菌活性。总之，本研究证实了LPJZ - 658的安全性和益生菌特性，表明它可作为人和动物应用的潜在益生菌候选物。
