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人体样本DNA浓度与生物电阻抗测量的相关性：一项初步研究。

Correlation of the DNA Concentration of Human Samples to Electrical Bioimpedance Measurements: A Pilot Study.

作者信息

Hernández-Salinas César A, Corzo-Cruz Alejandro, Sánchez-Monroy Virginia, Munguía-Cervantes Jacobo E, González-Díaz César A

机构信息

Centro Militar de Ciencias de la Salud - Escuela Militar de Graduados de Sanidad, UDEFA, CDMX, México.

Instituto Politécnico Nacional - Escuela Superior de Medicina, CDMX, México.

出版信息

J Electr Bioimpedance. 2023 Jan 14;13(1):132-135. doi: 10.2478/joeb-2022-0018. eCollection 2022 Jan.

DOI:10.2478/joeb-2022-0018
PMID:36694879
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9837867/
Abstract

It is necessary to evaluate the total deoxyribonucleic acid (DNA) concentration in gene expression assays. The existing techniques require equipment that is expensive for many labs in developing countries. Portable and inexpensive equipment is needed for easy and economical DNA quantification. Electrical bioimpedance spectroscopy (EBiS) is a non-invasive and inexpensive technique for examining the electrical properties of biological materials. The aim of this study was to explore a potential correlation between the measurement of total DNA extracted from human samples by UV-Vis spectrophotometry and EBiS. Hence, after quantifying the total DNA extracted from each sample by UV-Vis spectroscopy, EBiS was recorded and a possible correlation between the two measurements was analyzed. Considering the bioimpedance phase parameter at 5.24 MHz, a significant correlation was found with total DNA, especially when the concentration was below 100 ng/μL (Spearman coefficient = 0.82, p<0.005). Additional experiments are warranted to confirm these findings.

摘要

在基因表达分析中，评估总脱氧核糖核酸（DNA）浓度是很有必要的。现有技术所需的设备对于许多发展中国家的实验室来说成本高昂。因此需要便携且价格低廉的设备，以便轻松且经济地进行DNA定量分析。生物电阻抗光谱法（EBiS）是一种用于检测生物材料电学特性的非侵入性且廉价的技术。本研究的目的是探究通过紫外可见分光光度法从人类样本中提取的总DNA测量值与生物电阻抗光谱法之间的潜在相关性。因此，在用紫外可见光谱法定量每个样本中提取的总DNA后，记录生物电阻抗光谱法测量结果，并分析这两种测量结果之间可能存在的相关性。考虑5.24 MHz时的生物阻抗相位参数，发现其与总DNA存在显著相关性，尤其是当浓度低于100 ng/μL时（斯皮尔曼系数 = 0.82，p<0.005）。需要进行更多实验来证实这些发现。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cfa/9837867/81abd4362e09/joeb-13-132-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cfa/9837867/85bc124e9cad/joeb-13-132-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cfa/9837867/720a339e3f33/joeb-13-132-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cfa/9837867/954b722dd7a5/joeb-13-132-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cfa/9837867/81abd4362e09/joeb-13-132-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cfa/9837867/85bc124e9cad/joeb-13-132-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cfa/9837867/720a339e3f33/joeb-13-132-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cfa/9837867/954b722dd7a5/joeb-13-132-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cfa/9837867/81abd4362e09/joeb-13-132-g004.jpg

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本文引用的文献

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Multilaboratory assessment of variations in spectrophotometry-based DNA quantity and purity indexes.基于分光光度法的DNA数量和纯度指标变化的多实验室评估
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