Department of Engineering, University of Cambridge, Cambridge CB3 0FA, UK.
Sci Rep. 2013;3:2730. doi: 10.1038/srep02730.
Herein, we describe a novel integrated biosensor for performing dielectric spectroscopy to analyze biological samples. We analyzed biomolecule samples with different concentrations and demonstrated that the solution's impedance is highly correlated with the concentration, indicating that it may be possible to use this sensor as a concentration sensor. In contrast with standard spectrophotometers, this sensor offers a low-cost and purely electrical solution for the quantitative analysis of biomolecule solutions. In addition to determining concentrations, we found that the sample solution impedance is highly correlated with the length of the DNA fragments, indicating that the sizes of PCR products could be validated with an integrated chip-based, sample-friendly system within a few minutes. The system could be the basis of a rapid, low-cost platform for DNA characterization with broad applications in cancer and genetic disease research.
在这里，我们描述了一种用于进行介电谱分析以分析生物样本的新型集成生物传感器。我们分析了具有不同浓度的生物分子样本，并证明溶液的阻抗与浓度高度相关，这表明该传感器可能可用作浓度传感器。与标准分光光度计相比，该传感器为生物分子溶液的定量分析提供了一种低成本且纯电气的解决方案。除了确定浓度外，我们还发现样品溶液的阻抗与 DNA 片段的长度高度相关，这表明可以在几分钟内使用基于集成芯片的、对样品友好的系统验证 PCR 产物的大小。该系统可以作为快速、低成本的 DNA 特征分析平台的基础，在癌症和遗传疾病研究中有广泛的应用。
