Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G2H7, Canada.
J Proteome Res. 2023 Feb 3;22(2):454-461. doi: 10.1021/acs.jproteome.2c00620. Epub 2023 Jan 25.
CaspSites is a free-to-use database and web application for experimentally observed human caspase substrates using N-terminomics. It can be accessed and used by all users at the web URL www.caspsites.org. CaspSites stores cleavage site information identified for human caspases 1-9 in lysates and apoptotic cells, collected from their corresponding published studies. The database can be queried, viewed, and exported using the search page of the web application. The main parameters offered are protein substrate, cleavage site (P4-P4') residues, and individual caspase data sets, which can be connected using OR, AND, or NOT logical operators for custom user-built queries. CaspSites will be regularly updated with new experimental findings for understudied caspases, providing researchers insight into the distinctive roles human caspases play in cellular processes by identifying their target proteins in relation to each other.
CaspSites 是一个免费使用的数据库和网络应用程序，用于使用 N 端组学实验观察到的人类半胱天冬酶底物。所有用户均可通过 web 网址 www.caspsites.org 访问和使用 CaspSites。CaspSites 存储了从相应已发表的研究中收集的人源半胱天冬酶 1-9 在裂解物和凋亡细胞中鉴定的切割位点信息。可以使用网络应用程序的搜索页面查询、查看和导出数据库。该数据库提供的主要参数是蛋白质底物、切割位点（P4-P4'）残基和单个半胱天冬酶数据集，可以使用 OR、AND 或 NOT 逻辑运算符连接，用于自定义用户构建的查询。CaspSites 将定期更新关于研究较少的半胱天冬酶的新实验结果，通过确定彼此相关的靶蛋白，为研究人员深入了解人类半胱天冬酶在细胞过程中所扮演的独特角色提供了帮助。
