Spence C D, Coghlan J P, Denton D A, Gomez-Sanchez C, Mills E H, Whitworth J A, Scoggins B A
Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Australia.
J Steroid Biochem. 1987 Oct;28(4):441-3. doi: 10.1016/0022-4731(87)91064-8.
18-Oxo-cortisol (18-oxo-F) has been isolated from the urine of subjects with primary aldosteronism. This study examines the pressor, mineralocorticoid and glucocorticoid effects of 18-oxo-F in conscious sheep--a well studied species for the assessment of the pressor effect of steroid hormones. 18-oxo-F (24 mg/day i.v. for 5 days, n = 3) increased mean arterial pressure MAP (64 +/- 2 mmHg control and 75 +/- 6 mmHg on day 5 P less than 0.001). There was no change in heart rate. Plasma [K+] decreased from a control of 4.3 +/- 0.1 mmol/l control to 2.9 +/- 0.3 mmol/l on day 5 (P less than 0.001). Urinary Na+ excretion decreased on the first infusion day (233 +/- 18 mmol/day control and 124 +/- 20 mmol/day on infusion day 1 P less than 0.001). Urinary K+ excretion was reduced on days 1, 4 and 5 of the infusion. Thus in sheep, 18-oxo-F increased blood pressure associated with in vivo evidence of mineralocorticoid activity.
18-氧代皮质醇（18-oxo-F）已从原发性醛固酮增多症患者的尿液中分离出来。本研究检测了18-氧代皮质醇对清醒绵羊的升压作用、盐皮质激素作用和糖皮质激素作用——绵羊是一种在评估类固醇激素升压作用方面得到充分研究的物种。18-氧代皮质醇（静脉注射24毫克/天，共5天，n = 3）使平均动脉压（MAP）升高（对照时为64±2毫米汞柱，第5天时为75±6毫米汞柱，P<0.001）。心率没有变化。血浆[K⁺]从对照时的4.3±0.1毫摩尔/升降至第5天时的2.9±0.3毫摩尔/升（P<0.001）。在首次输注日，尿Na⁺排泄量减少（对照时为233±18毫摩尔/天，输注第1天时为124±20毫摩尔/天，P<0.001）。在输注的第1天、第4天和第5天，尿K⁺排泄量减少。因此，在绵羊中，18-氧代皮质醇升高血压，并伴有盐皮质激素活性的体内证据。
