Sharov V G, Saks V A, Kupriyanov V V, Lakomkin V L, Kapelko V I, Javadov S A
USSR Cardiology Research Center, Moscow.
J Thorac Cardiovasc Surg. 1987 Nov;94(5):749-61.
A phosphorus 31-nuclear magnetic resonance method was used to study the effect of exogenous phosphocreatine on the isolated perfused rat heart. The hearts were chemically arrested by St. Thomas' Hospital solution and made totally ischemic for 35 minutes at 37 degrees C. In the presence of phosphocreatine, 10 mmol/L, during ischemia, almost complete recovery of heart function and phosphocreatine content and 61% recovery of adenosine triphosphate content were observed after 30 minutes of postischemic reperfusion; in the control experiments without phosphocreatine, contractile function, intracellular phosphocreatine, and adenosine triphosphate contents were restored to 33%, 43%, and 26% of their normal values, respectively. Ultrastructural studies with a lanthanum tracer method showed remarkable protection of sarcolemma against ischemic injury by exogenous phosphocreatine at the level of the glycocalyx.
采用磷31核磁共振方法研究外源性磷酸肌酸对离体灌流大鼠心脏的影响。心脏用圣托马斯医院溶液进行化学停搏，并在37℃下完全缺血35分钟。在缺血期间，存在10 mmol/L磷酸肌酸的情况下，缺血后再灌注30分钟后，观察到心脏功能和磷酸肌酸含量几乎完全恢复，三磷酸腺苷含量恢复61%；在无磷酸肌酸的对照实验中，收缩功能、细胞内磷酸肌酸和三磷酸腺苷含量分别恢复至其正常值的33%、43%和26%。用镧示踪法进行的超微结构研究表明，外源性磷酸肌酸在糖萼水平对肌膜具有显著的抗缺血损伤保护作用。
