Keinänen M, Knuutila S, Bloomfield C D, Elonen E, de la Chapelle A
Blood. 1986 May;67(5):1240-3.
To determine the hematopoietic cell lineage of mitotic cells in human bone marrow on direct examination and after 24-hour culture, marrow mitoses from four healthy individuals were studied, using a new technique that allows analysis of karyotypes in cells whose cell membrane and cytoplasm have been preserved. Mitoses were identified as being of erythroid lineage by immunofluorescent staining for surface glycophorin A and as being of granulocytic lineage by cytoplasmic staining for Sudan black B. On direct marrow examination without prior culture, the great majority of mitoses (74% to 90%) were of erythroid lineage; only a few (0% to 10%) were granulocytic. After 24-hour culture, the percentage of erythroid mitoses (15% to 40%) decreased, while the percentage of granulocytic mitoses (58% to 87%) increased strikingly. These data indicate that mitotic cells of different hematopoietic cell lineages predominate in marrow at different culture times and offer a plausible explanation for the high frequency of normal karyotypes in acute myeloid leukemia after direct marrow cytogenetic evaluation.
为了在直接检查以及24小时培养后确定人类骨髓中有丝分裂细胞的造血细胞谱系，我们使用一种新技术对来自四名健康个体的骨髓有丝分裂进行了研究，该技术能够分析细胞膜和细胞质得以保留的细胞的核型。通过对表面血型糖蛋白A进行免疫荧光染色，有丝分裂被鉴定为红系谱系；通过对苏丹黑B进行细胞质染色，有丝分裂被鉴定为粒系谱系。在未经预先培养的直接骨髓检查中，绝大多数有丝分裂（74%至90%）为红系谱系；只有少数（0%至10%）为粒系。24小时培养后，红系有丝分裂的百分比（15%至40%）下降，而粒系有丝分裂的百分比（58%至87%）显著增加。这些数据表明，不同造血细胞谱系的有丝分裂细胞在不同培养时间在骨髓中占主导地位，并为直接骨髓细胞遗传学评估后急性髓系白血病中正常核型的高频率提供了一个合理的解释。
