School of Pharmacy, Guilin Medical University, Guilin, 541199, China.
Key Laboratory of Tumor Immunology and Microenvironmental Regulation, Guilin Medical University, Guilin, 541199, China.
J Cancer Res Clin Oncol. 2023 Aug;149(9):6203-6210. doi: 10.1007/s00432-022-04552-7. Epub 2023 Jan 26.
To investigate the synergistic effect of vitamin D and neferine on the growth and metastasis of colorectal cancer (CRC).
The synergistic effect of biologically active form of vitamin D, VD and neferine on the treatment of CRC was investigated by bliss analysis. Colony formation and wound healing ability, migration and invasion ability, and epithelial mesenchymal transition of HCT-116 cells, as a response to the combination treatment with VD and neferine were evaluated.
VD and neferine showed a synergistic effect on CRC cell growth at a relatively low dose. The wound healing and colony formation capacity, cell migration and invasion abilities were all decreased by combination use of VD and neferine, compared to the VD or neferine treated single group. Furthermore, VD and neferine significantly decreased the expressions of N-cadherin, vimentin, snail, and slug in HCT-116 cells.
These data suggest that neferine enhances the anticancer capability of VD and reduces the dose dependency of VD. The combination of vitamin D with neferine appears to be a potential therapeutic strategy for CRC.
研究维生素 D 和小檗碱对结直肠癌（CRC）生长和转移的协同作用。
通过 Bliss 分析研究生物活性形式的维生素 D（VD）和小檗碱联合治疗 CRC 的协同作用。评估 HCT-116 细胞对 VD 和小檗碱联合治疗的反应，包括集落形成和伤口愈合能力、迁移和侵袭能力以及上皮间质转化。
VD 和小檗碱在较低剂量下对 CRC 细胞生长表现出协同作用。与 VD 或小檗碱单药处理组相比，联合使用 VD 和小檗碱可降低伤口愈合和集落形成能力、细胞迁移和侵袭能力。此外，VD 和小檗碱可显著降低 HCT-116 细胞中 N-钙黏蛋白、波形蛋白、snail 和 slug 的表达。
这些数据表明，小檗碱增强了 VD 的抗癌能力，降低了 VD 的剂量依赖性。维生素 D 与小檗碱联合使用似乎是 CRC 的一种潜在治疗策略。
