NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.
J Med Virol. 2023 Feb;95(2):e28531. doi: 10.1002/jmv.28531.
Human rhinovirus (RV) is an important viral pathogen associated with severe acute respiratory tract infection. The present study retrospectively identified RV types in hospitalized patients with severe acute respiratory infection (SARI) from October 2017 to June 2019 in Henan Province, China. Real-time PCR was used to screen pharyngeal swab samples for RV. Then, the VP1 gene sequences of RV-positive samples were amplified and sequenced with nested primer PCR; subsequently, analyses of the molecular epidemiology and genetic diversity characteristics of the RV types were performed. Seventy-three out of 1015 respiratory samples were identified as RV-positive, from which 65 complete VP1 sequences were successfully sequenced. These RVs were classified into 41 different types, including 26 RV-A types, 2 RV-B types, and 13 RV-C types. The RVs showed an obvious seasonal distribution, with peaks in summer and autumn. The epidemic peak of RV-C was later than that of RV-A. In addition, two new types of species, B and C, were proposed, Bpat107 and Cpat58, respectively. Compared with other types in the same RV species, the pairwise nucleotide p-distances of the two novel RV types were 0.2620.402 and 0.2510.508, respectively. This study analyzed the seasonal and genetic characteristics of RV associated with SARI cases in Henan Province, China. Two novel RV types were proposed.
人类鼻病毒（RV）是一种与严重急性呼吸道感染相关的重要病毒病原体。本研究回顾性分析了 2017 年 10 月至 2019 年 6 月期间河南省住院严重急性呼吸道感染（SARI）患者中 RV 型别。采用实时 PCR 法对咽拭子样本进行 RV 筛查。然后，用巢式引物 PCR 扩增 RV 阳性样本的 VP1 基因序列，并对其进行测序；随后，对 RV 型别的分子流行病学和遗传多样性特征进行分析。从 1015 份呼吸道样本中鉴定出 73 份 RV 阳性样本，其中成功测序了 65 份完整的 VP1 序列。这些 RV 分为 41 种不同的型别，包括 26 种 RV-A 型、2 种 RV-B 型和 13 种 RV-C 型。RV 呈明显的季节性分布，夏季和秋季高峰。RV-C 的流行高峰晚于 RV-A。此外，还提出了两种新的种型，B 型和 C 型，分别为 Bpat107 和 Cpat58。与同一 RV 种的其他型别相比，两种新型 RV 型别的核苷酸 p-距离分别为 0.2620.402 和 0.2510.508。本研究分析了中国河南省 SARI 病例中与 RV 相关的季节性和遗传特征。提出了两种新的 RV 型别。
