Wang Y G, Xia B C, Xie Z B, Xu J, Zhang Y, Zhang Z B, Sun X, Wang H R, Wang H L, Kong Z, Song J H, Zhang Y D, Zhang Y
Medical School, Anhui University of Science and Technology, Huainan 232001, China.
National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Centers for Disease Control and Prevention, Beijing 102206, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2024 Feb 23;58:1-8. doi: 10.3760/cma.j.cn112150-20231207-00411.
To understand the infection status and molecular types of rhinovirus (RV) among cases of Acute Respiratory Infections (ARIs) in Luohe City, Henan Province, from 2017 to 2022. From October 2017 to June 2022, clinical and epidemiological data were collected from 2 270 cases of ARIs at Luohe Central Hospital in Henan Province. Throat swab specimens were obtained from these cases. Real-time quantitative polymerase chain reaction (qPCR) was used to screen for RV-positive specimens. Subsequently, the positive samples were subjected to nested reverse transcription polymerase chain reaction (nested RT-PCR) to amplify the full-length VP1 region. Using the MEGA software, along with 169 RV reference strains recommended by the International Committee on Taxonomy of Viruses, a phylogenetic tree was constructed to determine RV types. Among the 2 270 cases of ARIs, there were 1 283 male cases (56.52%). The median age (, ) was 3 (1, 6) years, with the population under 5 years old accounting for 68.59% (1 557/2 270). RV was detected in 137 cases (6.04%), of which 68 cases (49.64%) showed co-detection with other viruses, with the most common being co-detection with enterovirus, accounting for 14.60% (20/137). The RV detection rates in the age groups of 04 years, 514 years, 15~59 years, and≥60 years were 6.42% (100/1 557), 4.69% (21/448), 3.80% (6/158), and 9.35% (10/107), respectively, with no statistically significant differences (=5.310, =0.150). The overall detection rates of RV before (2017-2019) and during (2020-2022) the COVID-19 pandemic showed no statistically significant difference (=1.823, =0.177). A total of 109 VP1 sequences were obtained, including 62 types. Among them, RV-A, RV-B, and RV-C had 42, 3, and 17 types respectively. RV is one of the predominant pathogens in ARIs cases in Luohe City, Henan Province, from 2017 to 2022. Multiple types of RV co-circulate without any apparent dominant type.
为了解2017年至2022年河南省漯河市急性呼吸道感染（ARI）病例中鼻病毒（RV）的感染状况及分子类型。2017年10月至2022年6月，收集了河南省漯河市中心医院2270例ARI病例的临床和流行病学资料。从这些病例中获取咽拭子标本。采用实时定量聚合酶链反应（qPCR）筛选RV阳性标本。随后，对阳性样本进行巢式逆转录聚合酶链反应（巢式RT-PCR）扩增全长VP1区域。使用MEGA软件，结合国际病毒分类委员会推荐的169株RV参考毒株，构建系统发育树以确定RV类型。在2270例ARI病例中，男性病例有1283例（56.52%）。中位年龄（，）为3（1，6）岁，5岁以下人群占68.59%（1557/2270）。137例（6.04%）检测到RV，其中68例（49.64%）显示与其他病毒共同检测，最常见的是与肠道病毒共同检测，占14.60%（20/137）。0至4岁、5至14岁、15至59岁和≥60岁年龄组的RV检测率分别为6.42%（100/1557）、4.69%（21/448）、3.80%（6/158）和9.35%（10/107），差异无统计学意义（=5.310，=0.150）。COVID-19大流行之前（2017 - 2019年）和期间（2020 - 2022年）RV的总体检测率差异无统计学意义（=1.823，=0.177）。共获得109条VP1序列，包括62种类型。其中，RV - A、RV - B和RV - C分别有42种、3种和17种类型。RV是2017年至2022年河南省漯河市ARI病例中的主要病原体之一。多种类型的RV共同流行，没有明显的优势类型。
