van der Linden L, Bruning A H L, Thomas X V, Minnaar R P, Rebers S P H, Schinkel J, de Jong M D, Pajkrt D, Wolthers K C
Laboratory of Clinical Virology, Department of Medical Microbiology, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands.
Department of Paediatric Infectious Diseases, Emma Children's Hospital, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands.
Clin Microbiol Infect. 2016 Dec;22(12):1002.e9-1002.e14. doi: 10.1016/j.cmi.2016.08.007. Epub 2016 Aug 21.
Rhinoviruses (RVs) are frequently detected respiratory viruses that cause mild common cold symptoms, but may also lead to more severe respiratory tract infections. The large number of RV types, classified into species A, B and C, hampers clear insights into the epidemiology and clinical significance of each RV type. The aim of this study was to map the circulation of RV types in the Amsterdam area. RV-positive nasopharyngeal and oropharyngeal samples, collected from 2007 to 2012 in the Academic Medical Centre (Amsterdam, the Netherlands), were typed based on the sequence of the region coding for capsid proteins VP4 and VP2. RV-A, RV-B and RV-C were found in proportions of of 52.4% (334/637), 11.3% (72/637), and 36.2% (231/637), respectively. We detected 129 of the 167 currently classified types. RVs circulated throughout the entire year with a peak in the autumn and a decline in the summer. Some RV types were observed throughout the entire sampling period and others had a more seasonal pattern. Nine RV-A and four RV-B novel provisionally assigned types were identified. This study provides an insight into the molecular epidemiology of RVs in the Amsterdam area. The RVs circulating are diverse and include several provisionally new types.
鼻病毒（RVs）是常见的呼吸道病毒，可引起轻微的普通感冒症状，但也可能导致更严重的呼吸道感染。RVs有大量类型，分为A、B和C种，这妨碍了对每种RV类型的流行病学和临床意义的清晰认识。本研究的目的是描绘阿姆斯特丹地区RV类型的传播情况。2007年至2012年在学术医疗中心（荷兰阿姆斯特丹）采集的RV阳性鼻咽和口咽样本，根据衣壳蛋白VP4和VP2编码区域的序列进行分型。分别发现RV-A、RV-B和RV-C的比例为52.4%（334/637）、11.3%（72/637）和36.2%（231/637）。我们在目前分类的167种类型中检测到了129种。RVs全年传播，秋季达到高峰，夏季有所下降。在整个采样期间观察到一些RV类型，其他类型则有更明显的季节性模式。鉴定出9种新的RV-A和4种新的RV-B临时指定类型。本研究深入了解了阿姆斯特丹地区RVs的分子流行病学。传播的RVs种类多样，包括几种临时新类型。
