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核心技术专利：CN118964589B侵权必究
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负载淀粉的共价有机框架的高催化纳米酶-表面增强拉曼散射/吸收双模肽作为超痕量镉测定的生物传感器

Highly catalytic nanoenzyme of covalent organic framework loaded starch- surface-enhanced Raman scattering/absorption bi-mode peptide as biosensor for ultratrace determination of cadmium.

作者信息

Li Jingjing, Shu Yiyi, Li Chongning, Jiang Zhiliang

机构信息

School of Public Health, Guilin Medical University, Guilin, China.

Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin, China.

出版信息

Front Nutr. 2023 Jan 9;9:1075296. doi: 10.3389/fnut.2022.1075296. eCollection 2022.

DOI:10.3389/fnut.2022.1075296
PMID:36698455
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9870315/
Abstract

High affinity peptides (PTs) have been used in nanoanalysis, but there are no reports which combine PTs with a liquid crystal (LC) covalent organic framework (COF) supported soluble starch (SS) catalytic amplification system as a biosensor recognition element. In this study, a new, highly sensitive and selective bi-mode molecular biosensor has been developed for the determination of cadmium ion (Cd). Specifically, a highly catalytic and stable COF supported SS nanosol catalyst was fabricated such that a nanocatalytic indicator reaction system for HAuCl-sodium formate was established based on surface-enhanced Raman scattering (SERS). The Au nanoparticles produced exhibited a surface plasmon resonance (SPR) absorption peak at 535 nm and a SERS peak at 1,615 cm. Combining the nanocatalytic amplification indicator system with the specific PTs reaction permitted a sensitive and selective SERS/absorption bi-mode platform to be developed for the determination of cadmium in rice. The linear range for SERS determination was 0.025-0.95 nmol/L and the detection limit (DL) was 0.012 nmol/L.

摘要

高亲和力肽（PTs）已用于纳米分析，但尚无将PTs与液晶（LC）共价有机框架（COF）负载的可溶性淀粉（SS）催化扩增系统结合作为生物传感器识别元件的报道。在本研究中，开发了一种新型、高灵敏度和高选择性的双模式分子生物传感器用于测定镉离子（Cd）。具体而言，制备了一种高催化活性且稳定的COF负载SS纳米溶胶催化剂，基于表面增强拉曼散射（SERS）建立了用于HAuCl-甲酸钠的纳米催化指示反应体系。所产生的金纳米颗粒在535 nm处呈现表面等离子体共振（SPR）吸收峰，在1615 cm处呈现SERS峰。将纳米催化扩增指示体系与特异性PTs反应相结合，开发出一种灵敏且选择性的SERS/吸收双模式平台用于测定大米中的镉。SERS测定的线性范围为0.025 - 0.95 nmol/L，检测限（DL）为0.012 nmol/L。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bb4/9870315/1abd7c2b590c/fnut-09-1075296-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bb4/9870315/3e07eebc4733/fnut-09-1075296-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bb4/9870315/d98317ddf030/fnut-09-1075296-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bb4/9870315/d4b7212daa9f/fnut-09-1075296-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bb4/9870315/e3f98ce279bf/fnut-09-1075296-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bb4/9870315/1abd7c2b590c/fnut-09-1075296-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bb4/9870315/3e07eebc4733/fnut-09-1075296-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bb4/9870315/d98317ddf030/fnut-09-1075296-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bb4/9870315/d4b7212daa9f/fnut-09-1075296-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bb4/9870315/e3f98ce279bf/fnut-09-1075296-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bb4/9870315/1abd7c2b590c/fnut-09-1075296-g005.jpg

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引用本文的文献

[1]
Advances in Detection Technologies for Pesticide Residues and Heavy Metals in Rice: A Comprehensive Review of Spectroscopy, Chromatography, and Biosensors.

Foods. 2025-3-20

[2]
Research Progress on the Application of Covalent Organic Framework Nanozymes in Analytical Chemistry.

Biosensors (Basel). 2024-3-29

本文引用的文献

[1]
MXene catalytic amplification-fluorescence/absorption dimode aptamer sensor for the detection of trace Pb in milk.

Front Nutr. 2022-10-18

[2]
A time-resolved fluorescence lateral flow immunochromatographic assay based on oriented immobilized antibodies for the ultrasensitive detection of C-peptides in human serum.

Anal Chim Acta. 2022-5-22

[3]
A novel polypeptide-modified fluorescent gold nanoclusters for copper ion detection.

Sci Rep. 2022-4-22

[4]
Selective colorimetric detection of Cr(VI) using starch-stabilized silver nanoparticles and application for chromium speciation.

Spectrochim Acta A Mol Biomol Spectrosc. 2022-6-5

[5]
A novel aptamer RRS assay platform for ultratrace melamine based on COF-loaded Pd nanocluster catalytic amplification.

J Hazard Mater. 2022-2-5

[6]
Time-gated Raman spectroscopy and proteomics analyses of hypoxic and normoxic renal carcinoma extracellular vesicles.

Sci Rep. 2021-10-1

[7]
A nanoenzyme linked immunochromatographic sensor for rapid and quantitative detection of SARS-CoV-2 nucleocapsid protein in human blood.

Sens Actuators B Chem. 2021-12-15

[8]
A novel SPR based Fe@Ag core-shell nanosphere entrapped on starch matrix an optical probe for sensing of mercury(II) ion: A nanomolar detection, wide pH range and real water sample application.

Spectrochim Acta A Mol Biomol Spectrosc. 2021-12-15

[9]
A novel small molecular liquid crystal catalytic amplification-nanogold SPR aptamer absorption assay for trace oxytetracycline.

Talanta. 2021-10-1

[10]
Ratiometric fluorescent detection of silver nanoparticles in aqueous samples using peptide-based fluorogenic probes with aggregation-induced emission characteristics.

J Hazard Mater. 2021-6-5

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