基于 COF 负载 Pd 纳米簇催化扩增的新型适体 RRS 分析平台用于痕量三聚氰胺检测。
A novel aptamer RRS assay platform for ultratrace melamine based on COF-loaded Pd nanocluster catalytic amplification.
机构信息
State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin 541004, China; Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin 541004, China.
State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin 541004, China; Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin 541004, China.
出版信息
J Hazard Mater. 2022 Feb 5;423(Pt B):127263. doi: 10.1016/j.jhazmat.2021.127263. Epub 2021 Sep 20.
Two COFs of BzBD and BzBD loaded Pd nanoclusters (BzBD) were prepared using 1,3,5-benzenetricarboxaldehyde (Bz), benzidine (BD) and CO reducing agent, and were characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), infrared spectroscopy (IR) and other techniques. BzBD can strongly catalyze the new and stable Au@NiP nanoreaction that exhibit a strong resonance Rayleigh scattering (RRS) peak at 538 nm and a surface plasmon resonance (SPR) absorption peak at 395 nm, and the sensitive and facile RRS technique was used to study the indicator reaction. Combining the nanocatalytic amplification reaction with specific aptamer (Apt) of some target molecules such as melamine (ML), urea (UR) and bisphenol A (BPA), a simple, sensitive and selective Apt RRS assay platform was established. The linear range of the RRS detection platform for melamine is 0.0025-0.04 nmol/L, and the detection limit (DL) is 1.96 × 10 nmol/L. In addition, ML in real sample was analyzed, the stability of BzBD, BzBD, PdNPs and the catalytic mechanism of COF were also considered.
使用 1,3,5-苯三甲醛 (Bz)、联苯胺 (BD) 和 CO 还原剂制备了两种 BzBD 和 BzBD 负载钯纳米团簇 (BzBD) 的 COF，并通过透射电子显微镜 (TEM)、X 射线粉末衍射 (XRD)、红外光谱 (IR) 等技术进行了表征。BzBD 可以强烈催化新的和稳定的 Au@NiP 纳米反应，该反应在 538nm 处表现出强共振瑞利散射 (RRS) 峰，在 395nm 处表现出表面等离子体共振 (SPR) 吸收峰，并使用灵敏且易于操作的 RRS 技术研究了指示剂反应。通过将纳米催化放大反应与某些目标分子（如三聚氰胺 (ML)、尿素 (UR) 和双酚 A (BPA)）的特定适体 (Apt) 结合，建立了一种简单、灵敏和选择性的 Apt RRS 分析平台。RRS 检测平台对三聚氰胺的线性范围为 0.0025-0.04 nmol/L，检测限 (DL) 为 1.96×10 nmol/L。此外，还分析了实际样品中的 ML，考虑了 BzBD、BzBD、PdNPs 的稳定性和 COF 的催化机制。
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