Parandin Rahmatollah, Ghowsi Mahnaz, Dadbod Ahmad
Department of Biology, Payame Noor University, Tehran, Iran.
Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran.
Avicenna J Phytomed. 2023 Jan-Feb;13(1):7-17. doi: 10.22038/AJP.2022.20893.
Cyclophosphamide (CP)-induced testicular toxicity has been reported in recipient patients. The current study was designed to evaluate protective effects of hydroalcoholic extract of L. fruit (HARF) against CP-induced testicular toxicity in BALB/c mice.
Thirty-five mice were divided into five groups as follows: group I (control), group II (CP, received CP 100 mg/kg on days 1, 8, 15, and 22), group III (CP + HARF 250 mg/kg), group IV (CP + HARF 500 mg/kg), and group V (CP + HARF 750 mg/kg). In the groups III, IV, and V that received CP, the HARF was simultaneously administered via intraperitoneal injections for 28 consecutive days starting from day 1. On the 29 day, sperm parameters, stress oxidative biomarkers, and mRNA expression of in testis tissue, as well as blood testosterone were evaluated.
The CP exposure decreased sperm parameters, superoxide dismutase (SOD) activity, testosterone, and mRNA expression levels and increased the malondialdehyde (MDA). HARF at the dose of 500 mg/kg improved sperm count and viability and increased SOD and catalase activities, glutathione peroxidase (GPx) activity, testosterone level, and expression and reduced MDA. Also, HARF at the dose of 750 mg/kg improved sperm parameters and increased SOD, catalase, and GPx activities, total testosterone level, and expression, and reduced MDA in comparison with the CP group.
According to our findings, HARF at the doses of 500 and 750 mg/kg inhibited the ruinous effects of CP on the reproductive system in mice.
已有报道称接受环磷酰胺（CP）治疗的患者会出现睾丸毒性。本研究旨在评估L.果实水醇提取物（HARF）对BALB/c小鼠CP诱导的睾丸毒性的保护作用。
35只小鼠分为五组，如下：第一组（对照组），第二组（CP组，在第1、8、15和22天接受100 mg/kg的CP），第三组（CP + 250 mg/kg HARF），第四组（CP + 500 mg/kg HARF），第五组（CP + 750 mg/kg HARF）。在接受CP的第三、四和五组中，从第1天开始连续28天通过腹腔注射同时给予HARF。在第29天，评估精子参数、氧化应激生物标志物、睾丸组织中[具体物质]的mRNA表达以及血液睾酮水平。
CP暴露降低了精子参数、超氧化物歧化酶（SOD）活性、睾酮水平以及[具体物质]的mRNA表达水平，并增加了丙二醛（MDA）含量。500 mg/kg剂量的HARF改善了精子数量和活力，增加了SOD、过氧化氢酶活性、谷胱甘肽过氧化物酶（GPx）活性、睾酮水平以及[具体物质]的表达，并降低了MDA含量。此外，与CP组相比，750 mg/kg剂量的HARF改善了精子参数，增加了SOD、过氧化氢酶和GPx活性、总睾酮水平以及[具体物质]的表达，并降低了MDA含量。
根据我们的研究结果，500和750 mg/kg剂量的HARF可抑制CP对小鼠生殖系统的破坏作用。
