Sharma Divya, Baas Tracey, Nogales Aitor, Martinez-Sobrido Luis, Gromiha M Michael
Protein Bioinformatics Lab, Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600036, India.
Texas Biomedical Research Institute, San Antonio, TX 78227, USA.
Bioinform Adv. 2023 Jan 2;3(1):vbac102. doi: 10.1093/bioadv/vbac102. eCollection 2023.
We have developed a web-based tool, CoDe (Codon Deoptimization) that deoptimizes genetic sequences based on different codon usage bias, ultimately reducing expression of the corresponding protein. The tool could also deoptimize the sequence for a specific region and/or selected amino acid(s). Moreover, CoDe can highlight sites targeted by restriction enzymes in the wild-type and codon-deoptimized sequences. Importantly, our web-based tool has a user-friendly interface with flexible options to download results.
The web-based tool CoDe is freely available at https://web.iitm.ac.in/bioinfo2/codeop/landing_page.html.
Supplementary data are available at online.
我们开发了一种基于网络的工具CoDe（密码子去优化），它基于不同的密码子使用偏好对基因序列进行去优化，最终降低相应蛋白质的表达。该工具还可以针对特定区域和/或选定的氨基酸对序列进行去优化。此外，CoDe可以突出野生型和密码子去优化序列中限制性内切酶靶向的位点。重要的是，我们的基于网络的工具具有用户友好的界面，并有灵活的选项来下载结果。
基于网络的工具CoDe可在https://web.iitm.ac.in/bioinfo2/codeop/landing_page.html免费获取。
补充数据可在网上获取。
