Abbasi Mahmoud, Alsaikhan Fahad, Obaid Rasha Fadhel, Jahani Shohreh, Biroudian Saeed, Oveisee Maziar, Arab Mohammad Reza, Aramesh-Boroujeni Zahra, Foroughi Mohammad Mehdi
Medical Ethics and Law Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
College of Pharmacy, Prince Sattam Bin Abdulaziz University, Alkharj, Saudi Arabia.
Front Chem. 2023 Jan 9;10:1060706. doi: 10.3389/fchem.2022.1060706. eCollection 2022.
In the article presented herein, a deoxyribonucleic acid (DNA) biosensor is introduced for Vincristine determination in pharmaceutical preparations based on the modification of screen printed electrode (SPE) with double-stranded DNA (ds-DNA), polypyrrole (PP), peony-like CuO:Tb nanostructure (P-L CuO:Tb NS). The developed sensor indicated a wide linear response to Vincristine concentration ranged from 1.0 nM to 400.0 μM with a limit of detection as low as .21 nM. The intercalation of Vincristine with DNA guanine led to the response. The optimized parameters for the biosensor performance were ds-DNA/Vincristine interaction time, DNA concentration and type of buffer solution. The docking investigation confirm the minor groove interaction between guanine base at surface of or ds-DNA/PP/P-L CuO:Tb NS/SPE and Vincristine. The proposed sensor could successfully determine Vincristine in Vincristine injections and biological fluids, with acceptable obtains.
在本文介绍的文章中，基于用双链DNA（ds-DNA）、聚吡咯（PP）、牡丹状CuO:Tb纳米结构（P-L CuO:Tb NS）修饰丝网印刷电极（SPE），引入了一种用于测定药物制剂中长春新碱的脱氧核糖核酸（DNA）生物传感器。所开发的传感器对长春新碱浓度在1.0 nM至400.0 μM范围内呈现出宽线性响应，检测限低至0.21 nM。长春新碱与DNA鸟嘌呤的嵌入导致了该响应。生物传感器性能的优化参数为ds-DNA/长春新碱相互作用时间、DNA浓度和缓冲溶液类型。对接研究证实了ds-DNA/PP/P-L CuO:Tb NS/SPE表面的鸟嘌呤碱基与长春新碱之间的小沟相互作用。所提出的传感器能够成功测定长春新碱注射液和生物流体中的长春新碱，结果令人满意。
