Al-Qargholi Basim, Al-Dolaimy F, Altalbawy Farag M A, Kadhim Abed J, Alsaalamy Ali Hashiem, Suliman Muath, Abbas Ahmed Hussien R
Biomedical Engineering Department, Al-Mustaqbal University College, 51001 Hilla, Iraq.
Al-Zahraa University for Women, Karbala, Iraq.
Anal Methods. 2023 Oct 12;15(39):5146-5156. doi: 10.1039/d3ay01103h.
The present study developed a DNA biosensor to determine pemigatinib for the first time. Three-dimensional carnation flower-like Eu:β-MnO nanostructures (3D CF-L Eu:β-MnO NSs) and a screen-printed electrode (SPE) modified with polyaniline (PA) were employed. The double-stranded DNA was also immobilized completely on the PA/3D CF-L Eu:β-MnO NSs/SPE. Then, electrochemical techniques were used for characterizing the modified electrode. After that, the interaction between pemigatinib and DNA was shown by a reduction in the oxidation current of guanine using differential pulse voltammetry (DPV). According to the analysis, the dynamic range of pemigatinib was between 0.001 and 180.0 μM, indicating the new electrode has a low limit of detection (LOD = 0.23 nM) for pemigatinib. Afterwards, pemigatinib in real samples was measured using the PA/3D CF-L Eu:β-MnO NSs/SPE loaded with ds-DNA. The proposed DNA biosensor showed good selectivity toward pemigatinib in the presence of other interference analytes, such as other ions, structurally related pharmaceuticals, and plasma proteins. In addition, the interaction site of pemigatinib with DNA was predicted by molecular docking, which showed the interaction of pemigatinib with the guanine bases of DNA through a groove binding mode. Finally, we employed the -test to verify the capability of the ds-DNA/PA/3D CF-L Eu:β-MnO NSs/SPE for analyzing pemigatinib in real samples.
本研究首次开发了一种用于测定培米替尼的DNA生物传感器。采用了三维康乃馨花状铕掺杂β-二氧化锰纳米结构（3D CF-L Eu:β-MnO NSs）和用聚苯胺（PA）修饰的丝网印刷电极（SPE）。双链DNA也完全固定在PA/3D CF-L Eu:β-MnO NSs/SPE上。然后，用电化学技术对修饰电极进行表征。之后，通过差分脉冲伏安法（DPV）检测鸟嘌呤氧化电流的降低，显示了培米替尼与DNA之间的相互作用。分析表明，培米替尼的动态范围在0.001至180.0 μM之间，表明新电极对培米替尼具有低检测限（LOD = 0.23 nM）。随后，使用负载双链DNA的PA/3D CF-L Eu:β-MnO NSs/SPE测定实际样品中的培米替尼。所提出的DNA生物传感器在存在其他干扰分析物（如其他离子、结构相关药物和血浆蛋白）的情况下，对培米替尼表现出良好的选择性。此外，通过分子对接预测了培米替尼与DNA的相互作用位点，结果表明培米替尼通过沟槽结合模式与DNA的鸟嘌呤碱基相互作用。最后，我们采用t检验来验证ds-DNA/PA/3D CF-L Eu:β-MnO NSs/SPE分析实际样品中培米替尼的能力。
