Şener Raman Tuğçe, Kuehnert Mathias, Daikos Olesya, Scherzer Tom, Krömmelbein Catharina, Mayr Stefan G, Abel Bernd, Schulze Agnes
Institute of Surface Engineering (IOM), Leipzig, Germany.
Wilhelm-Ostwald-Institute for Physical and Theoretical Chemistry, Institute of Chemical Technology of the University Leipzig, Leipzig, Germany.
Front Chem. 2023 Jan 9;10:1094981. doi: 10.3389/fchem.2022.1094981. eCollection 2022.
Gelatin-based hydrogels are highly desirable biomaterials for use in wound dressing, drug delivery, and extracellular matrix components due to their biocompatibility and biodegradability. However, insufficient and uncontrollable mechanical properties and degradation are the major obstacles to their application in medical materials. Herein, we present a simple but efficient strategy for a novel hydrogel by incorporating the synthetic hydrogel monomer polyethylene glycol diacrylate (PEGDA, offering high mechanical stability) into a biological hydrogel compound (gelatin) to provide stable mechanical properties and biocompatibility at the resulting hybrid hydrogel. In the present work, PEGDA/gelatin hybrid hydrogels were prepared by electron irradiation as a reagent-free crosslinking technology and without using chemical crosslinkers, which carry the risk of releasing toxic byproducts into the material. The viscoelasticity, swelling behavior, thermal stability, and molecular structure of synthesized hybrid hydrogels of different compound ratios and irradiation doses were investigated. Compared with the pure gelatin hydrogel, 21/9 wt./wt. % PEGDA/gelatin hydrogels at 6 kGy exhibited approximately up to 1078% higher storage modulus than a pure gelatin hydrogel, and furthermore, it turned out that the mechanical stability increased with increasing irradiation dose. The chemical structure of the hybrid hydrogels was analyzed by Fourier-transform infrared (FTIR) spectroscopy, and it was confirmed that both compounds, PEGDA and gelatin, were equally present. Scanning electron microscopy images of the samples showed fracture patterns that confirmed the findings of viscoelasticity increasing with gelatin concentration. Infrared microspectroscopy images showed that gelatin and PEGDA polymer fractions were homogeneously mixed and a uniform hybrid material was obtained after electron beam synthesis. In short, this study demonstrates that both the presence of PEGDA improved the material properties of PEGDA/gelatin hybrid hydrogels and the resulting properties are fine-tuned by varying the irradiation dose and PEGDA/gelatin concentration.
基于明胶的水凝胶因其生物相容性和生物可降解性，是用于伤口敷料、药物递送和细胞外基质成分的理想生物材料。然而，机械性能不足且不可控以及降解问题是其在医用材料应用中的主要障碍。在此，我们提出了一种简单而有效的策略来制备新型水凝胶，即将合成水凝胶单体聚乙二醇二丙烯酸酯（PEGDA，具有高机械稳定性）掺入生物水凝胶化合物（明胶）中，以使所得的杂化水凝胶具有稳定的机械性能和生物相容性。在本研究中，PEGDA/明胶杂化水凝胶是通过电子辐照制备的，这是一种无试剂交联技术，无需使用化学交联剂，因为化学交联剂存在向材料中释放有毒副产物的风险。研究了不同化合物比例和辐照剂量的合成杂化水凝胶的粘弹性、溶胀行为、热稳定性和分子结构。与纯明胶水凝胶相比，6 kGy下21/9 wt./wt.%的PEGDA/明胶水凝胶的储能模量比纯明胶水凝胶高出约1078%，此外，结果表明机械稳定性随辐照剂量的增加而提高。通过傅里叶变换红外（FTIR）光谱分析了杂化水凝胶的化学结构，证实PEGDA和明胶这两种化合物均存在。样品的扫描电子显微镜图像显示的断裂模式证实了粘弹性随明胶浓度增加而增加的结果。红外显微光谱图像表明，明胶和PEGDA聚合物组分均匀混合，电子束合成后得到了均匀的杂化材料。简而言之，本研究表明，PEGDA的存在改善了PEGDA/明胶杂化水凝胶的材料性能，并且通过改变辐照剂量和PEGDA/明胶浓度可以微调所得性能。
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