Bioorganic & Biophysical Chemistry Laboratory, Linnaeus University Centre for Biomaterials Chemistry, Department of Chemistry & Biomedical Sciences, Linnaeus University, SE-391 82, Kalmar, Sweden.
Department of Chemistry, School of Applied Sciences and Humanities, Vignan's Foundation for Science, Technology and Research (Deemed to Be University), Vadlamudi, Guntur, 522 213, Andhra Pradesh, India.
Talanta. 2023 May 1;256:124298. doi: 10.1016/j.talanta.2023.124298. Epub 2023 Jan 21.
Proteinaceous, tunable nanostructures of zein (prolamine of corn) were developed as biotinyl-specific receptors using a molecular imprinting technique. Sacrificial templates, such as latex beads (LB3) and anodized alumina membrane (AAM), have been used to control nanostructural patterns in biotin-imprinted zein (BMZ). Briefly, a methanolic solution of the zein-biotin complex was drop cast upon a self-organized LB3 and AAM templates on Au/quartz surfaces. Subsequent dissolution of these sacrificial templates affords highly oriented, predetermined, and uniformly grown hyperporous (300 nm) and nanowires (150 nm) motifs of zein (BMZ-LB3 and BMZ-AAM), as shown by scanning electron microscopy (SEM). Selective extraction of biotin molecular template cast-off site-selective biotin imprints within these zein nanostructures complementary to biotinyl moieties. Alternatively, biotin-imprinted zein nanoparticles (BMZ-Np) and thin film (BMZ-MeOH) were prepared by coacervation and drop casting methods, respectively. Density functional theoretical (DFT) studies reveal strong hydrogen-bonded interaction of biotin with serine and glutamine residues of zein. Quartz crystal microbalance (QCM) studies show remarkable sensitivity of the hyperporous-BMZ-LB3 and nanowires of BMZ-AAM towards biotin derivative (biotin methyl ester) by five (24.75 ± 1.34 Hz/mM) and four (18.19 ± 0.75 Hz/mM) times, respectively, higher than the BMZ-MeOH. Enhanced permeability features of the zein nanostructures, when templated with LB3, enable the QCM detection of biotin- or its derivatives down to 12.9 ng mL from dairy products (Kefir). The outcome of this study shall be a key aspect in interfacing biological materials with micro-/nano-sensors and electronic devices for detecting pertinent analytes using sustainably developed biopolymer-based nanostructures.
采用分子印迹技术，开发了玉米醇溶蛋白（玉米醇溶蛋白）的蛋白状、可调纳米结构作为生物素特异性受体。牺牲模板，如乳胶珠（LB3）和阳极氧化铝膜（AAM），已被用于控制生物素印迹玉米醇溶蛋白（BMZ）的纳米结构模式。简而言之，将玉米醇溶蛋白-生物素复合物的甲醇溶液滴铸在 Au/石英表面上自组织的 LB3 和 AAM 模板上。随后溶解这些牺牲模板，可获得高度定向、预定和均匀生长的超多孔（300nm）和纳米线（150nm）图案的玉米醇溶蛋白（BMZ-LB3 和 BMZ-AAM），如扫描电子显微镜（SEM）所示。在这些玉米醇溶蛋白纳米结构中，选择性地提取生物素分子模板留下的位置选择性生物素印迹，与生物素基部分互补。或者，通过凝聚和滴铸方法分别制备生物素印迹的玉米醇溶蛋白纳米颗粒（BMZ-Np）和薄膜（BMZ-MeOH）。密度泛函理论（DFT）研究表明，生物素与玉米醇溶蛋白的丝氨酸和谷氨酰胺残基之间存在强氢键相互作用。石英晶体微天平（QCM）研究表明，超多孔-BMZ-LB3 和 BMZ-AAM 的纳米线对生物素衍生物（生物素甲酯）具有显著的敏感性，其灵敏度分别比 BMZ-MeOH 高五倍（24.75±1.34Hz/mM）和四倍（18.19±0.75Hz/mM）。LB3 模板化的玉米醇溶蛋白纳米结构具有增强的渗透性特征，可使 QCM 检测到乳制品（开菲尔）中低至 12.9ng/mL 的生物素或其衍生物。这项研究的结果将是将生物材料与微/纳米传感器和电子设备接口的关键方面，用于使用可持续开发的基于生物聚合物的纳米结构检测相关分析物。
