Department of General Surgery, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai 201399, China.
Institute of Translational Medicine, Shanghai University, Shanghai 200444, China.
Tissue Cell. 2023 Apr;81:102032. doi: 10.1016/j.tice.2023.102032. Epub 2023 Jan 22.
Ellagic acid (EA), found in fruits and foods, has been shown to be effective in the treatment of breast, colon and bladder cancer. However, due to the complexity of colon cancer, the therapeutic mechanism of EA for colon cancer is still unclear. Cell Counting Kit-8 (CCK-8) assay were employed to investigate the cell proliferation. Western blotting and flow cytometry assays were utilized to investigate apoptosis and autophagy in CRC cells (HCT116), respectively. Moreover, western blotting and luciferase reporter assays were evaluated the effect of EA on AMPK/mTOR pathway. Through flow cytometry analysis, EA could promote the apoptosis of HCT116 cells. In addition, EA can reduce the phosphorylation of mTOR, promoted phosphorylation of AMPK, and induced autophagy in HCT116 cells. Also, Dorsomorphin pretreatment can reduce the expression of autophagy protein, which indicates that EA induces autophagy through AMPK/mTOR pathway. These results suggest that EA inhibits the growth of colon cancer through AMPK/mTOR pathway and induces apoptosis and protective autophagy.
鞣花酸（EA）存在于水果和食物中，已被证明可有效治疗乳腺癌、结肠癌和膀胱癌。然而，由于结肠癌的复杂性，EA 治疗结肠癌的机制仍不清楚。细胞计数试剂盒-8（CCK-8）检测法用于研究细胞增殖。Western blot 和流式细胞术检测分别用于研究 CRC 细胞（HCT116）中的细胞凋亡和自噬。此外，Western blot 和荧光素酶报告基因检测评估了 EA 对 AMPK/mTOR 通路的影响。通过流式细胞术分析，EA 可促进 HCT116 细胞的凋亡。此外，EA 可以降低 mTOR 的磷酸化，促进 AMPK 的磷酸化，并诱导 HCT116 细胞自噬。另外，Dorsomorphin 预处理可以降低自噬蛋白的表达，表明 EA 通过 AMPK/mTOR 通路诱导自噬。这些结果表明，EA 通过 AMPK/mTOR 通路抑制结肠癌的生长，并诱导细胞凋亡和保护性自噬。
