Environmental Protection and Control Program, Eskisehir Osmangazi University, 26110 Eskisehir, Turkey; Department of Biotechnology and Biosafety, Graduate School of Natural and Applied Sciences, Eskisehir Osmangazi University, 26040 Eskisehir, Turkey.
Department of Biotechnology and Biosafety, Graduate School of Natural and Applied Sciences, Eskisehir Osmangazi University, 26040 Eskisehir, Turkey.
Bioresour Technol. 2023 Mar;372:128669. doi: 10.1016/j.biortech.2023.128669. Epub 2023 Jan 23.
For cost-competitive biosynthesis of polyhydroxybutyrate (PHB), the screening of efficient producers and characterization of their genomic potential is fundamental. In this study, 94 newly isolated halophilic strains from Turkish salterns were screened for their polyhydroxyalkanoates (PHAs) biosynthesis capabilities through fermentation. Halomonas halmophila 18H was found to be the highest PHB producer, yielding 63.72 % of its biomass as PHB. The PHB produced by this strain was physically and chemically characterized using various techniques. Its genome was also sequenced and found to be large (6,713,657 bp) and have a GC content of 59.9 %. Halomonas halmophila 18H was also found to have several copies of PHB biosynthesis genes, as well as 20 % more protein-coding genes and 1075 singletons compared to other high PHB producers. These unique genomic features make it a promising cell factory for the simultaneous production of PHAs and other biotechnologically important secondary metabolites.
为了在具有成本竞争力的条件下合成聚羟基丁酸酯（PHB），筛选高效生产菌并对其基因组潜力进行表征至关重要。在这项研究中，通过发酵，从土耳其盐田筛选了 94 株新分离的嗜盐菌，以评估其聚羟基烷酸酯（PHA）的生物合成能力。发现盐单胞菌 18H 是 PHB 的最高生产菌，其生物量中有 63.72%转化为 PHB。使用各种技术对该菌株产生的 PHB 进行了物理和化学表征。还对其基因组进行了测序，发现其基因组较大（6713657bp），GC 含量为 59.9%。此外，盐单胞菌 18H 还具有多个 PHB 生物合成基因，与其他高 PHB 生产菌相比，其编码蛋白的基因多 20%，单基因多 1075 个。这些独特的基因组特征使其成为同时生产 PHAs 和其他生物技术重要次生代谢物的有前途的细胞工厂。
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