Department of Physiological Sciences, Federal University of Espirito Santo, Vitória, Brazil.
Mastology Service Evangelical Hospital of Vila Velha, Vila Velha, Brazil.
Appl Spectrosc. 2023 Apr;77(4):405-417. doi: 10.1177/00037028231156194. Epub 2023 Mar 9.
Breast cancer is a heterogeneous disease, and its spread involves a succession of clinical and pathological stages. Screening is predominantly based on mammography, which has critical limitations related to the effectiveness and production of false-positive or false-negative results, generating discomfort and low adherence. In this context, infrared with attenuated total reflection Fourier transform infrared (ATR FT-IR) spectroscopy emerges as a non-destructive sample tool, which is non-invasive, label-free, has a low operating-cost, and requires only a small amount of sample, including liquid plasma samples. We sought to evaluate the clinical applicability of ATR FT-IR in breast cancer screening. ATR FT-IR spectroscopy through its highest potential spectral biomarker could distinguish, by liquid plasma biopsy, breast cancer patients and healthy controls, obtaining a sensitivity of 97%, specificity of 93%, a receiver operating characteristic ROC curve of 97%, and a prediction accuracy of 94%. The main variance between the groups was mainly in the band 1511 cm of the control group, 1502 and 1515 cm of the cancer group, which are the peaks of the bands referring to proteins and amide II. ATR FT-IR spectroscopy has demonstrated to be a promising tool for breast cancer screening, given its time efficiency, cost of approach, and its high ability to distinguish between the liquid plasma samples of breast cancer patients and healthy controls.
乳腺癌是一种异质性疾病，其扩散涉及一系列临床和病理阶段。筛查主要基于乳房 X 光摄影，它具有与有效性和产生假阳性或假阴性结果相关的关键限制，导致不适和低依从性。在这种情况下，衰减全反射傅里叶变换红外（ATR FT-IR）光谱的红外线作为一种无损样本工具出现，它是非侵入性的、无标记的、具有低运营成本，并且只需要少量样本，包括液体血浆样本。我们试图评估 ATR FT-IR 在乳腺癌筛查中的临床适用性。ATR FT-IR 光谱通过其最高潜在光谱生物标志物，可以通过液体血浆活检区分乳腺癌患者和健康对照者，获得 97%的灵敏度、93%的特异性、97%的接收器操作特征 ROC 曲线和 94%的预测准确性。组间的主要差异主要在对照组的 1511 cm 波段、癌症组的 1502 和 1515 cm 波段，这些是指蛋白质和酰胺 II 带的峰。ATR FT-IR 光谱已被证明是一种有前途的乳腺癌筛查工具，因为它具有时间效率、接近成本和区分乳腺癌患者和健康对照者的液体血浆样本的高能力。
