Crop Protection Area, Department of Agronomy, Universidade Federal Rural de Pernambuco, 52171-900, Recife, PE, Brazil.
Department of Genetics, Universidade Federal de Pernambuco, 50670-901, Recife, PE, Brazil.
Phytopathology. 2023 Jul;113(7):1360-1364. doi: 10.1094/PHYTO-08-22-0279-A. Epub 2023 Aug 26.
Cashew () angular leaf spot is caused by pigmented and non-pigmented strains of pv. , which have been isolated from infected plants in Brazil. The disease symptoms can be observed in leaves, stems, and fruits. Given that infection in young fruits results in fruits unsuitable for commercialization, angular leaf spot represents a serious threat to the cashew crop in Brazil. Here, we report the genomic sequencing of seven pigmented strains of pv. , obtained from the leaves of cashew trees from São Paulo state, Brazil, in 2009. The construction of the libraries was carried out according to the manufacturer, and whole-genome sequencing was performed using the Illumina HiSeq 2500 platform. Genome size, number of coding sequences, largest contig length, and N50 ranged from 4,996,984 to 5,003,485 bp, 4,621 to 4,643 bp, 212,513 to 362,232 bp, and 113,582 to 141,003 bp, respectively. GC content and RNA numbers were 64.68% and 54, respectively, for all strains. ANIm and DDH analyses showed values above 99.5 and 92.1% among these strains and the non-pigmented pathotype strain of pv. (IBSBF2579). A maximum likelihood tree built with 2,708 core genes grouped all pv. strains in the same clade, with a 100% bootstrap. These resources will contribute in a relevant way to help understand the ecological, taxonomic, evolutionary, pathogenicity, and virulence aspects of pv. , which will be useful for the study and development of techniques for managing cashew angular leaf spot.
腰果角斑病是由色素和非色素型 pv. 引起的，这些菌株已从巴西感染植物中分离出来。该病害症状可在叶片、茎和果实上观察到。由于幼果感染会导致果实不适于商业化，因此腰果角斑病对巴西的腰果作物构成严重威胁。在这里，我们报告了 2009 年从巴西圣保罗州腰果树上采集的七个色素型 pv. 菌株的基因组测序结果。文库构建根据制造商进行，全基因组测序使用 Illumina HiSeq 2500 平台进行。基因组大小、编码序列数量、最大连续序列长度和 N50 范围分别为 4,996,984 到 5,003,485 bp、4,621 到 4,643 bp、212,513 到 362,232 bp 和 113,582 到 141,003 bp。所有菌株的 GC 含量和 RNA 数量分别为 64.68%和 54。ANI 和 DDH 分析表明，这些菌株之间的数值均高于 99.5%，与非色素型 pv. （IBSBF2579）的数值也高于 92.1%。使用 2,708 个核心基因构建的最大似然树将所有 pv. 菌株聚在同一分支中，支持率为 100%。这些资源将有助于深入了解 pv. 的生态、分类、进化、致病性和毒力等方面，这对于研究和开发腰果角斑病管理技术将非常有用。
