Houthoff H J, Poppema S, Ebels E J, Elema J D
Acta Neuropathol. 1978 Dec 15;44(3):203-10. doi: 10.1007/BF00691068.
Fifteen primary intracranial reticulum cell sarcomas and five cases with an additional solitary extracranial tumor mass have been studied. For comparison, seven extracranial malignant non-Hodgkin lymphomas and normal lymphoid tissue were included. The methods used on formalin-fixed paraffin-embedded tissue sections were an immunoperoxidase technique for the demonstration of intracellular immunoglobulins, microglial staining, Gomori's reticulin, methylgreen-pyronin, Giemsa, diastase resistant PAS, Mallory's PTAH and H&E. Electron microscopy was performed in one primary brain tumor. According to histopathologic criteria all tumors could be classified as malignant non-Hodgkin lymphomas, predominantly of the pleomorphic immunocytic or of the immunoblastic type; follicular lymphomas were notably absent. In all cases intracellular immunoglobulins were demonstrable in tumor cells and in a majority of the tumors these were monoclonal. Thus, all malignant lymphomas proved to be of B cell origin with demonstrable cytoplasmic immunoglobulin production. Based on the microglial staining more than half of the malignant lymphomas could also be classified as microgliomas. As a comparable staining was present in non-Hodgkin lymphomas outside the CNS, microglioma characteristics are not associated with intracranial growth.
我们对15例原发性颅内网状细胞肉瘤以及5例伴有额外孤立性颅外肿瘤块的病例进行了研究。作为对照，纳入了7例颅外恶性非霍奇金淋巴瘤和正常淋巴组织。对福尔马林固定石蜡包埋的组织切片采用的方法包括：用于显示细胞内免疫球蛋白的免疫过氧化物酶技术、小胶质细胞染色、Gomori网状纤维染色、甲基绿-派洛宁染色、吉姆萨染色、抗淀粉酶PAS染色、马洛里磷钨酸苏木精染色和苏木精-伊红染色。对1例原发性脑肿瘤进行了电子显微镜检查。根据组织病理学标准，所有肿瘤均可归类为恶性非霍奇金淋巴瘤，主要为多形性免疫细胞型或免疫母细胞型；滤泡性淋巴瘤明显缺失。在所有病例中，肿瘤细胞内均可显示细胞内免疫球蛋白，且大多数肿瘤中的免疫球蛋白为单克隆性。因此，所有恶性淋巴瘤均被证实起源于B细胞，可产生可显示的细胞质免疫球蛋白。基于小胶质细胞染色，超过半数的恶性淋巴瘤也可归类为小胶质细胞瘤。由于中枢神经系统外的非霍奇金淋巴瘤也存在类似的染色，因此小胶质细胞瘤的特征与颅内生长无关。
