Abdulfattah Shaimaa Y, Samawi Farah T
Medical and Molecular Department, Biotechnology Research Center Al-Nahrain University, Jadriya Baghdad Iraq.
Health Sci Rep. 2023 Jan 23;6(1):e1073. doi: 10.1002/hsr2.1073. eCollection 2023 Jan.
Urinary tract infection (UTI) is the most common infection in type 2 diabetes patients. TNF-β is a cytokine with multiple functions in immunomodulatory and inflammatory mechanisms. The variation at position +252 A/G of TNF-β impacts both gene expression and plasma concentration of TNF-β proteins. The findings may shed light on the genetic factors that predispose diabetic patients in Iraq to UTIs.
A total of 200 individuals were divided into 100 patients with type 2 diabetes, categorized according to UTI, and 100 control subjects. Genetic analysis of +252 A/G of the TNF-β gene was carried out using the TaqMan probe allele discrimination method. The level of TNF-β was estimated by the ELISA technique.
In the recessive model (GG vs. AA/AG) of TNF-β + 252 A/G in T2D/UTI patients compared to controls, a significant association = 0.029 (OR: 2.8; CI 95% = 1.14-7.09): E = 15.6% was observed. Furthermore, in T2D patients without UTI, the dominant model AA versus AG/GG was associated with a preventive role P: 31.3% (OR: 0.4; CI 95% = 0.22-0.88) and a value = (0.02). Overall, AG proportions showed a high level of TNF-β within the control group = 0.03, while all proportions of the +252 A/G showed significant differences in TNF-β level between groups ≤ 0.05. Pearson's correlation analysis observed a link between TNF- levels, fasting plasma glucose (FPG), and HbA1c.
In T2D patients, the G allele may be linked to a higher probability of UTI, as well as an increased level of TNF-β in a genotype-dependent manner.
尿路感染（UTI）是2型糖尿病患者中最常见的感染。肿瘤坏死因子-β（TNF-β）是一种在免疫调节和炎症机制中具有多种功能的细胞因子。TNF-β基因+252位A/G的变异会影响TNF-β蛋白的基因表达和血浆浓度。这些发现可能有助于揭示伊拉克糖尿病患者易患UTI的遗传因素。
总共200人被分为100例2型糖尿病患者（根据UTI进行分类）和100例对照受试者。使用TaqMan探针等位基因鉴别方法对TNF-β基因的+252 A/G进行基因分析。通过酶联免疫吸附测定（ELISA）技术评估TNF-β水平。
在2型糖尿病/UTI患者中，与对照组相比，TNF-β +252 A/G的隐性模型（GG与AA/AG）存在显著关联，P = 0.029（比值比：2.8；95%置信区间= 1.14 - 7.09）：观察到E = 15.6%。此外，在无UTI的2型糖尿病患者中，显性模型AA与AG/GG具有预防作用，P：31.3%（比值比：0.4；95%置信区间= 0.22 - 0.88），P值=（0.02）。总体而言，对照组中AG比例显示TNF-β水平较高，P = 0.03，而+252 A/G的所有比例在组间TNF-β水平上均显示出显著差异，P≤0.05。Pearson相关性分析观察到TNF-水平、空腹血糖（FPG）和糖化血红蛋白（HbA1c）之间存在关联。
在2型糖尿病患者中，G等位基因可能与UTI的较高概率相关，并且以基因型依赖的方式导致TNF-β水平升高。
