Yamashita K, Iwamoto T, Iijima S
Acta Pathol Jpn. 1978 Sep;28(5):689-95. doi: 10.1111/j.1440-1827.1978.tb00908.x.
Lysozyme activity of macrophages and giant cells in various human granulomas were examined with immunoperoxidase bridge method in tissue sections. Various numbers of epithelioid cells and giant cells of epithelioid cell granulomas of tuberculosis, sarcoidosis and Crohn's disease exhibited intense granular cytoplasmic lysozyme activity. Foreign body granulomas induced with various substances showed negative or faintly positive lysozyme stain. Macrophages and giant cells of aspergillus granuloma associated with thymus hypoplasia and T-cell depression contained no lysozyme. The results suggest that cell-mediated immunology plays an important role for the lysozyme synthesis of macrophages in granuloma.
采用免疫过氧化物酶桥法对组织切片中各种人类肉芽肿内巨噬细胞和巨细胞的溶菌酶活性进行了检测。结核、结节病和克罗恩病的上皮样细胞肉芽肿中有数量不等的上皮样细胞和巨细胞呈现强烈的颗粒状胞质溶菌酶活性。由各种物质诱发的异物肉芽肿溶菌酶染色呈阴性或弱阳性。与胸腺发育不全和T细胞抑制相关的曲霉菌肉芽肿中的巨噬细胞和巨细胞不含溶菌酶。结果表明，细胞介导的免疫在肉芽肿内巨噬细胞溶菌酶合成中起重要作用。
