Center for Interdisciplinary Research in Biology (CIRB), College de France, CNRS, INSERM, Université PSL, Paris, France.
Sorbonne Université, CNRS, Laboratoire de Biologie du Développement de Villefranche-sur-mer (LBDV), Villefranche-sur-Mer 06230, France.
Sci Adv. 2023 Jan 27;9(4):eadd2873. doi: 10.1126/sciadv.add2873.
During meiosis, DNA recombination allows the shuffling of genetic information between the maternal and paternal chromosomes. Recombination is initiated by double-strand breaks (DSBs) catalyzed by the conserved enzyme Spo11. How this crucial event is connected to other meiotic processes is unexpectedly variable depending on the species. Here, we knocked down by CRISPR in the jellyfish . Germ cells in mutants fail to assemble synaptonemal complexes and chiasmata, and in consequence, homologous chromosome pairs in females remain unassociated during oocyte growth and meiotic divisions, creating aneuploid but fertilizable eggs that develop into viable larvae. thus shares an ancient eukaryotic dependence of synapsis and chromosome segregation on Spo11-generated DSBs. Phylogenetically, belongs to Cnidaria, the sister clade to Bilateria where classical animal model species are found, so these results provide fresh evolutionary perspectives on meiosis regulation.
在减数分裂过程中，DNA 重组允许母源和父源染色体之间的遗传信息交换。重组由保守酶 Spo11 催化的双链断裂 (DSB) 引发。这个关键事件如何与其他减数分裂过程相关联，根据物种的不同而存在显著的差异。在这里，我们通过 CRISPR 在海蜇中敲低 。 突变体的生殖细胞无法组装联会复合体和交叉，结果是，在卵母细胞生长和减数分裂过程中，雌性的同源染色体对仍然没有关联，形成非整倍体但可受精的卵子，这些卵子发育成可育的幼虫。 因此，它与 Spo11 产生的 DSB 引发的联会和染色体分离在古老的真核生物中具有依赖性。从系统发生学上讲， 属于刺胞动物门，是后生动物的姐妹群，后生动物中存在经典的动物模式物种，因此这些结果为减数分裂调控提供了新的进化视角。
