Foster Samuel W, Parker Deklin, Piccolo Christopher, Will Matthew, Grinias James P
Department of Chemistry & Biochemistry, Rowan University, 201 Mullica Hill Rd., NJ, 08028, Glassboro, USA.
Anal Bioanal Chem. 2023 Jul;415(18):4147-4152. doi: 10.1007/s00216-023-04564-3. Epub 2023 Jan 28.
Emitter tip arrays for electrospray ionization have been used for a variety of MS sample introduction purposes, including detection of multiple sample eluent streams and improved accuracy through parallel infusion of an internal standard. User control is typically required for targeted application of high voltage to specific channels to maximize analyte signal and minimize other background signals. In this communication, an automated approach to applying electrospray voltage only when a detectable analyte is present is described. An in-line absorbance detector is used to identify the presence of an analyte in the fluidic path between the sample introduction valve and the mass spectrometer. A Raspberry Pi-controlled system is then used to apply high voltage to a downstream emitter tip at the MS inlet following a delay volume between the detectors. Demonstration of this technique on two parallel sample channels is reported, including a pulsed voltage application to maximize signal when analytes elute on each channel simultaneously.
用于电喷雾电离的发射器尖端阵列已被用于多种质谱样品引入目的，包括检测多个样品洗脱液流以及通过内标物的平行注入提高准确性。通常需要用户控制向特定通道施加高电压，以最大化分析物信号并最小化其他背景信号。在本通讯中，描述了一种仅在存在可检测分析物时才施加电喷雾电压的自动化方法。使用在线吸光度检测器来识别样品引入阀和质谱仪之间流体路径中分析物的存在。然后，在检测器之间的延迟体积之后，使用树莓派控制系统向质谱仪入口处的下游发射器尖端施加高电压。报道了在两个平行样品通道上对该技术的演示，包括在每个通道上的分析物同时洗脱时施加脉冲电压以最大化信号。
