Haggarty Jennifer, Oppermann Madalina, Dalby Matthew J, Burchmore Richard J, Cook Ken, Weidt Stefan, Burgess Karl E V
Polyomics, University of Glasgow, 211 Wolfson Wohl Translational Cancer Research Centre, Garscube Campus, Glasgow, G61 1QH UK.
Thermo Fisher Scientific, Hemel Hempstead, UK.
Metabolomics. 2015;11(5):1465-1470. doi: 10.1007/s11306-014-0770-7. Epub 2015 Jan 11.
The serial coupling of a reversed-phase liquid chromatography (RPLC) column to a hydrophilic interaction liquid chromatography (HILIC) column has been developed in recent years for the detection of polar and nonpolar metabolites. TCA intermediates, bile acid standards and numerous polar and non-polar metabolites extracted from beer were analysed using a combined RPLC/HILIC method. Non-polar metabolites were retained by the RPLC column. Polar metabolites not retained by the RPLC column were retained and separated by the HILIC column. The results from this study validate this simple yet powerful metabolomics approach.
近年来，反相液相色谱（RPLC）柱与亲水作用液相色谱（HILIC）柱的串联联用技术已被开发用于检测极性和非极性代谢物。采用RPLC/HILIC联用方法分析了三羧酸循环（TCA）中间体、胆汁酸标准品以及从啤酒中提取的众多极性和非极性代谢物。非极性代谢物被RPLC柱保留。未被RPLC柱保留的极性代谢物则被HILIC柱保留并分离。本研究结果验证了这种简单却强大的代谢组学方法。
