Yeboaa Comfort, Odoi Hayford, Owusu Ntim Rhoda, Boakye Yaw Duah, Kwakye-Nuako Godwin, Agyare Christian, Boamah Vivian Etsiapa, Badu Kingsley
Pharmaceutical Microbiology Section, Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Department of Pharmaceutical Microbiology, School of Pharmacy, University of Health and Allied Sciences, Ho, Ghana.
Arch Microbiol. 2023 Jan 28;205(2):74. doi: 10.1007/s00203-023-03411-4.
Leishmaniasis is a vector-borne disease caused by an intracellular protozoan parasite. The presence of secondary bacterial infections in cutaneous leishmaniasis wounds exacerbate lesion development and could lead to delay in the healing process. This study sought to determine the resistance patterns of bacteria co-infecting cutaneous leishmaniasis wounds from selected communities in the Nkwanta district. Various bacteria were isolated and characterized from exudates obtained from wound swabs collected with sterile cotton tipped applicators. Confirmation of bacterial identity was done using the analytical profile index and the matrix-assisted laser desorption/ionization time of flight mass spectrometry. Antibiotic susceptibility tests were performed using agar disc diffusion method according to the Clinical and Laboratory Standards Institute breakpoint values. A total of eleven (11) secondary bacterial species (spp) were isolated from the 33 wound samples that tested positive for Leishmania kinetoplast DNA, among which Staphylococcus aureus was the most predominant (31%). The pathogenic bacteria that colonized the wounds included Bacillus subtilis (23.8%), Pantoea species (11.9%), Klebsiella pneumoniea (7.1%), Enterobacter cloacae (7.1%), Aeromonas species (4.8%), Serratia marcescens (4.8%), Serratia liquefacien (2.4%), Serratia plymutheca (2.4%), Providencia rettgeri (2.4%) and Cronobacter species (2.4%). Most of the isolates were resistant to beta-lactam antibiotics and the third-generation cephalosporin. Notably, 84.6% of the S. aureus isolates were methicillin and ciprofloxacin resistant whilst 92.3% were resistant to ampicillin. About sixty-nine percent (69.2%) showed intermediate susceptibility to Erythromycin. Additionally, S. plymutheca was resistant to all the test antibiotics. This study suggests colonization of cutaneous leishmaniasis wounds with varied bacterial species that are mostly resistant to beta-lactam group of antibiotics.
利什曼病是一种由细胞内原生动物寄生虫引起的媒介传播疾病。皮肤利什曼病伤口中继发性细菌感染的存在会加剧病变发展，并可能导致愈合过程延迟。本研究旨在确定来自恩夸塔区选定社区的皮肤利什曼病伤口中共感染细菌的耐药模式。从用无菌棉签收集的伤口拭子渗出物中分离并鉴定了各种细菌。使用分析谱指数和基质辅助激光解吸/电离飞行时间质谱法对细菌身份进行确认。根据临床和实验室标准协会的断点值，采用琼脂纸片扩散法进行抗生素敏感性试验。从33份检测利什曼原虫动基体DNA呈阳性的伤口样本中，共分离出11种继发性细菌，其中金黄色葡萄球菌最为常见（31%）。定植在伤口的病原菌包括枯草芽孢杆菌（23.8%）、泛菌属（11.9%）、肺炎克雷伯菌（7.1%）、阴沟肠杆菌（7.1%）、气单胞菌属（4.8%）、粘质沙雷氏菌（4.8%）、液化沙雷氏菌（2.4%）、普城沙雷氏菌（2.4%）、雷氏普罗威登斯菌（2.4%）和阪崎肠杆菌属（2.4%）。大多数分离株对β-内酰胺类抗生素和第三代头孢菌素耐药。值得注意的是，84.6%的金黄色葡萄球菌分离株对甲氧西林和环丙沙星耐药，而92.3%对氨苄西林耐药。约69.2%对红霉素表现出中度敏感性。此外，普城沙雷氏菌对所有测试抗生素均耐药。本研究表明，皮肤利什曼病伤口定植有多种细菌，这些细菌大多对β-内酰胺类抗生素耐药。
