Future Industries Institute, University of South Australia, GPO Box 2471, Adelaide, SA 5001, Australia.
Future Industries Institute, University of South Australia, GPO Box 2471, Adelaide, SA 5001, Australia.
J Chromatogr A. 2023 Feb 22;1691:463813. doi: 10.1016/j.chroma.2023.463813. Epub 2023 Jan 20.
Over the past two decades significant technical advancement in the field of western blotting has been made possible through the utilization of microfluidic technologies. In this review we provide a critical overview of these advancements, highlighting the advantages and disadvantages of each approach. Particular attention is paid to the development of now commercially available systems, including those for single cell analysis. This review also discusses more recent developments, including algorithms for automation and/or improved quantitation, the utilization of different materials/chemistries, use of projection electrophoresis, and the development of triBlots. Finally, the review includes commentary on future advances in the field based on current developments, and the potential of these systems for use as point-of-care devices in healthcare.
在过去的二十年中，通过微流控技术的应用，使蛋白质印迹技术领域取得了重大技术进步。在这篇综述中，我们对这些进展进行了批判性的概述，强调了每种方法的优缺点。特别关注现在商业化系统的发展，包括单细胞分析系统。本文还讨论了最近的进展，包括自动化和/或改进定量算法的发展、不同材料/化学物质的利用、投影电泳的使用以及三印迹技术的发展。最后，根据当前的发展情况，对该领域的未来进展进行了评论，并探讨了这些系统在医疗保健中用作即时检测设备的潜力。
