Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; Biomedical Research Institute, Shenzhen-PKU-HKUST Medical Center, Shenzhen 518057, China.
J Chromatogr A. 2015 Jan 16;1377:13-26. doi: 10.1016/j.chroma.2014.12.041. Epub 2014 Dec 18.
Rapid and timely diagnosis of infectious diseases is a critical determinant of clinical outcomes and general public health. For the detection of various pathogens, microfluidics-based platforms offer many advantages, including speed, cost, portability, high throughput, and automation. This review provides an overview of the recent advances in microfluidic technologies for point-of-care (POC) diagnostics for infectious diseases. The key aspects of such technologies for the development of a fully integrated POC platform are introduced, including sample preparation, on-chip nucleic acid analysis and immunoassay, and system integration/automation. The current challenges to practical implementation of this technology are discussed together with future perspectives.
快速、及时地诊断传染病是临床疗效和公众健康的关键决定因素。对于各种病原体的检测，基于微流控的平台具有许多优势，包括速度、成本、便携性、高通量和自动化。本综述概述了用于即时诊断（POC）传染病的微流控技术的最新进展。介绍了此类技术在开发完全集成的 POC 平台方面的关键方面，包括样品制备、芯片上核酸分析和免疫分析以及系统集成/自动化。讨论了将该技术实际应用的当前挑战以及未来展望。
