Polito Joshua T, Lange B Markus
Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, Pullman, WA, United States.
Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, Pullman, WA, United States; Dewey Scientific LLC, Pullman, WA, United States.
Methods Enzymol. 2023;680:381-419. doi: 10.1016/bs.mie.2022.07.029. Epub 2022 Sep 6.
Terpenes are the primary determinants of cannabis flower aroma, and ongoing research tests their potential for impacting the overall experience. Frustratingly, despite the importance of terpenes in cannabis physiology and commercial uses, literature reports vary widely regarding the major constituents of volatile blends and the concentrations of individual terpenes. In this article, we provide detailed descriptions of complementary approaches that will allow researchers to determine the identity and quantity of cannabis terpenes unequivocally and reliably. These standard operating procedures will guide decisions about which method to employ to address specific analytical goals. We are including two application examples to illustrate the utility of different approaches for tackling the analysis of terpenes in cannabis flower samples.
萜类化合物是大麻花朵香气的主要决定因素，目前的研究正在测试它们对整体体验的影响潜力。令人沮丧的是，尽管萜类化合物在大麻生理学和商业用途中很重要，但关于挥发性混合物的主要成分和单个萜类化合物的浓度，文献报道差异很大。在本文中，我们详细描述了一些互补方法，这些方法将使研究人员能够明确且可靠地确定大麻萜类化合物的种类和数量。这些标准操作规程将指导关于采用哪种方法来实现特定分析目标的决策。我们还纳入了两个应用示例，以说明不同方法在处理大麻花朵样品中萜类化合物分析方面的实用性。
