Mendez Carolina Bobadilla, Gonda Amber, Shah Jay V, Siebert Jake N, Zhao Xinyu, He Shuqing, Riman Richard E, Tan Mei Chee, Moghe Prabhas V, Ganapathy Vidya, Pierce Mark C
Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854 USA.
Engineering Product Development, Singapore University of Technology and Design, Singapore 487372, Singapore.
IEEE J Sel Top Quantum Electron. 2021 Sep-Oct;27(5). doi: 10.1109/jstqe.2021.3066895. Epub 2021 Mar 17.
Fluorescence-guided surgery (FGS) is an emerging technique for tissue visualization during surgical procedures. Structures of interest are labeled with exogenous probes whose fluorescent emissions are acquired and viewed in real-time with optical imaging systems. This study investigated rare-earth-doped albumin-encapsulated nanocomposites (REANCs) as short-wave infrared emitting contrast agents for FGS. Experiments were conducted using an animal model of 4T1 breast cancer. The signal-to-background ratio (SBR) obtained with REANCs was compared to values obtained using indocyanine green (ICG), a near-infrared dye used in clinical practice. Prior to resection, the SBR for tumors following intratumoral administration of REANCs was significantly higher than for tumors injected with ICG. Following FGS, evaluation of fluorescence intensity levels in excised tumors and at the surgical bed demonstrated higher contrast between tissues at these sites with REANC contrast than ICG. REANCs also demonstrated excellent photostability over 2 hours of continuous illumination, as well as the ability to perform FGS under ambient lighting, establishing these nanocomposites as a promising contrast agent for FGS applications.
荧光引导手术（FGS）是一种在手术过程中用于组织可视化的新兴技术。感兴趣的结构用外源性探针标记，其荧光发射通过光学成像系统实时采集和观察。本研究调查了稀土掺杂白蛋白包裹的纳米复合材料（REANCs）作为用于FGS的短波红外发射造影剂。使用4T1乳腺癌动物模型进行实验。将REANCs获得的信噪比（SBR）与使用吲哚菁绿（ICG，临床实践中使用的近红外染料）获得的值进行比较。在切除前，瘤内注射REANCs后肿瘤的SBR显著高于注射ICG的肿瘤。FGS后，对切除肿瘤和手术床处荧光强度水平的评估表明，与ICG相比，使用REANC造影时这些部位的组织之间具有更高的对比度。REANCs在连续照明2小时内还表现出优异的光稳定性，以及在环境光下进行FGS的能力，确立了这些纳米复合材料作为FGS应用中有前景的造影剂的地位。
