从用过的BinaxNOW新冠病毒抗原自测盒中回收RNA后进行的新型冠状病毒2型分子检测和全基因组测序。
SARS-CoV-2 molecular testing and whole genome sequencing following RNA recovery from used BinaxNOW COVID-19 Antigen Self Tests.
作者信息
Nguyen Phuong-Vi, Carmola Ludy Registre, Wang Ethan, Bassit Leda, Rao Anuradha, Greenleaf Morgan, Sullivan Julie A, Martin Greg S, Lam Wilbur A, Waggoner Jesse J, Piantadosi Anne
出版信息
medRxiv. 2023 Jan 9:2023.01.09.23284337. doi: 10.1101/2023.01.09.23284337.
Widespread use of over-the-counter rapid diagnostic tests for SARS-CoV-2 has led to a decrease in availability of clinical samples for viral genomic surveillance. As an alternative sample source, we evaluated RNA isolated from BinaxNOW swabs stored at ambient temperature for SARS-CoV-2 rRT-PCR and full viral genome sequencing. 81 of 103 samples (78.6%) yielded detectable RNA, and 46 of 57 samples (80.7 %) yielded complete genome sequences. Our results illustrate that SARS-CoV-2 RNA extracted from used Binax test swabs provides an important opportunity for improving SARS-CoV-2 genomic surveillance, evaluating transmission clusters, and monitoring within-patient evolution.
SARS-CoV-2的非处方快速诊断测试的广泛使用导致用于病毒基因组监测的临床样本可用性下降。作为替代样本来源，我们评估了从在室温下保存的BinaxNOW拭子中分离的RNA用于SARS-CoV-2逆转录聚合酶链反应（rRT-PCR）和完整病毒基因组测序的情况。103个样本中有81个（78.6%）产生了可检测到的RNA，57个样本中有46个（80.7%）产生了完整的基因组序列。我们的结果表明，从用过的Binax测试拭子中提取的SARS-CoV-2 RNA为改善SARS-CoV-2基因组监测、评估传播集群以及监测患者体内病毒演变提供了重要机会。
Zotero 插件