Emory University Department of Medicine, Atlanta, GA, USA; Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA, USA.
Emory University Department of Pathology and Laboratory Medicine, Atlanta, GA, USA.
J Clin Virol. 2023 May;162:105426. doi: 10.1016/j.jcv.2023.105426. Epub 2023 Mar 24.
Widespread use of over-the-counter rapid diagnostic tests for SARS-CoV-2 has led to a decrease in availability of clinical samples for viral genomic surveillance. As an alternative sample source, we evaluated RNA isolated from BinaxNOW swabs stored at ambient temperature for SARS-CoV-2 rRT-PCR and full viral genome sequencing. 81 of 103 samples (78.6%) yielded detectable RNA, and 46 of 57 samples (80.7 %) yielded complete genome sequences. Our results illustrate that SARS-CoV-2 RNA extracted from used Binax test swabs provides an important opportunity for improving SARS-CoV-2 genomic surveillance, evaluating transmission clusters, and monitoring within-patient evolution.
广泛使用非处方快速诊断检测 SARS-CoV-2 导致用于病毒基因组监测的临床样本可用性降低。作为替代样本来源，我们评估了从常温保存的 BinaxNOW 拭子中分离的 RNA 用于 SARS-CoV-2 rRT-PCR 和全病毒基因组测序。103 个样本中有 81 个（78.6%）可检测到 RNA，57 个样本中有 46 个（80.7%）可获得完整的基因组序列。我们的结果表明，从使用过的 Binax 检测拭子中提取的 SARS-CoV-2 RNA 为改善 SARS-CoV-2 基因组监测、评估传播集群和监测患者内病毒演变提供了重要机会。
