Mishra Birendra K, Das Sujit, Nandy Suman K, Patel Maulik, Hati Subrota
Department of Rural Development and Agricultural Production, North-Eastern Hill University, Tura Campus, Chasingre, Meghalaya 794002 India.
Biotechnology Industry Research Assistance Council, New Delhi, Delhi 110003 India.
J Food Sci Technol. 2023 Feb;60(2):504-516. doi: 10.1007/s13197-022-05633-8. Epub 2022 Nov 29.
The probiotic attributes and genomic profiles of amylase-producing strains from rice-based fermented foods of Meghalaya in the North-Eastern India were evaluated in the study. A preliminary screening of 17 lactic acid bacteria strains was performed based on their starch hydrolysis and glucoamylase activities. Out of 17 strains, 5 strains ( KGL4, RNS4, WTS4, KGL2, and KGL3A) were selected for further characterization of different probiotic attributes. Whole-genome sequencing of two of the best strains was carried out using a shotgun sequencing platform based on their rich probiotic attributes. The EPS production was in the range of 2.89-3.92 mg/mL. KGL2 (41.5%) and KGL3A (41%) showed the highest antioxidant activity. The highest antibiotic susceptibility was exhibited by all the five strains against ampicillin, ranging from 24.66 to 27.33 mm. The lactobacilli isolates used in the study could survive the simulated gastric/intestinal juices. Genomic characterization of KGL4 and KGL3A illustrated their possible adherence to the intestinal wall, specialized metabolic patterns, and possible role in boosting host immunity.
The online version contains supplementary material available at 10.1007/s13197-022-05633-8.
在本研究中，对印度东北部梅加拉亚邦米基发酵食品中产淀粉酶菌株的益生菌特性和基因组概况进行了评估。基于淀粉水解和糖化酶活性对17株乳酸菌进行了初步筛选。在这17株菌株中，选择了5株（KGL4、RNS4、WTS4、KGL2和KGL3A）进一步表征不同的益生菌特性。基于它们丰富的益生菌特性，使用鸟枪法测序平台对其中两株最佳菌株进行了全基因组测序。胞外多糖产量在2.89 - 3.92毫克/毫升范围内。KGL2（41.5%）和KGL3A（41%）表现出最高的抗氧化活性。所有五株菌株对氨苄青霉素的抗生素敏感性最高，范围为24.66至27.33毫米。本研究中使用的乳酸杆菌分离株能够在模拟胃液/肠液中存活。KGL4和KGL3A的基因组特征表明它们可能附着于肠壁、具有特殊的代谢模式以及在增强宿主免疫力方面可能发挥的作用。
在线版本包含可在10.1007/s13197 - 022 - 05633 - 8获取的补充材料。
