Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan, 173234, India.
Department of Microbiology, School of Interdisciplinary and Applied Life Sciences Central University of Haryana, Mahendergarh, Haryana, 123031, India.
Probiotics Antimicrob Proteins. 2019 Sep;11(3):774-784. doi: 10.1007/s12602-018-9466-z.
The present study aimed to isolate and identify the potential probiotic lactic acid bacteria (LAB) from traditionally fermented wheat flour dough known as "babroo." A total of 26 lactic isolates were obtained from 50 different samples collected from different locations of the Himalayan province (Himachal Pradesh). To ascertain non-pathogenic nature, the isolates were screened by hemolytic activity leading to 20 non-hemolytic strains which were subjected to tolerance to simulated gastrointestinal conditions. Seven different strains were selected based on their tolerance to gastrointestinal conditions. All the selected seven isolates were found to be susceptible to ampicillin and resistant to gentamicin. The phylogenetic analysis revealed three different strains of Lactobacillus fermentum, two strains of Lactobacillus plantarum, and two of Pediococcus acidilactici. The isolates exhibit different degrees of cell surface hydrophobicity (20.0-42.0%), cellular autoaggregation (5.0-41.0%), and adhesion to mucin (35.0-78.0%). The different lactic strains also possessed higher antioxidant properties (87.6-92.9%) determined in terms of free radical scavenging activity, bile salt hydrolase activity, cholesterol lowering activity (49.0-75.0%), and exopolysaccharide production. The principal component analysis (PCA) on probiotic attributes indicated that L. plantarum K90, L. fermentum K75, and L. fermentum K78 can be used as potential probiotic candidates.
本研究旨在从传统发酵的小麦面团（称为“babroo”）中分离和鉴定潜在的益生菌乳酸菌（LAB）。从喜马偕尔邦（印度北部一邦）不同地区采集的 50 个不同样本中获得了 26 个乳酸菌分离株。为了确定非致病性，通过溶血活性筛选分离株，导致 20 株非溶血株，然后对其进行模拟胃肠道条件的耐受性筛选。根据对胃肠道条件的耐受性，从 20 株非溶血株中选择了 7 个不同的菌株。所有选定的 7 个分离株均对氨苄青霉素敏感，对庆大霉素耐药。系统发育分析显示，有 3 株不同的发酵乳杆菌、2 株植物乳杆菌和 2 株嗜酸乳球菌。这些分离株表现出不同程度的细胞表面疏水性（20.0-42.0%）、细胞自动聚集性（5.0-41.0%）和对粘蛋白的粘附性（35.0-78.0%）。不同的乳酸菌株还具有较高的抗氧化特性（87.6-92.9%），这是通过自由基清除活性、胆盐水解酶活性、降低胆固醇活性（49.0-75.0%）和胞外多糖生产来衡量的。益生菌特性的主成分分析（PCA）表明，L. plantarum K90、L. fermentum K75 和 L. fermentum K78 可用作潜在的益生菌候选株。
