Liu Gengsong, Liu Aijing, Yang Cheng, Zhou Congcong, Zhou Qiaoyan, Li Haizhu, Yang Hongchun, Mo Jiahao, Zhang Zhidan, Li Gonghe, Si Hongbin, Ou Changbo
College of Animal Science and Technology, Guangxi University, Nanning, China.
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
Front Microbiol. 2023 Jan 11;13:1076154. doi: 10.3389/fmicb.2022.1076154. eCollection 2022.
continues to be one of the most important pathogens capable of causing a wide range of infections in different sites of the body in humans and livestock. With the emergence of methicillin-resistant strains and the introduction of strict laws on antibiotic usage in animals, antibiotic replacement therapy has become increasingly popular. Previous studies have shown that L. extract exerts a certain degree of bacteriostatic effect, although the active ingredients are unknown. In the present study, the antibacterial activity of the organic acid of (OAPO) against was examined using a series of experiments, including the minimum inhibitory concentration, growth curve, and bacteriostasis curve. antibacterial mechanisms were evaluated based on the integrity and permeability of the cell wall and membrane, scanning electron microscopy, and soluble protein content. A mouse skin wound recovery model was used to verify the antibacterial effects of OAPO on . The results showed that OAPO not only improved skin wound recovery but also decreased the bacterial load in skin wounds. Moreover, the number of inflammatory cells and cytokines decreased in the OAPO-treated groups. In summary, this study reports a botanical extract that can inhibit and , indicating the potential use of OAPO to prevent and control infection in the near future.
仍然是能够在人类和牲畜身体的不同部位引起广泛感染的最重要病原体之一。随着耐甲氧西林菌株的出现以及动物抗生素使用严格法律的出台，抗生素替代疗法越来越受欢迎。先前的研究表明，L.提取物具有一定程度的抑菌作用，尽管其活性成分尚不清楚。在本研究中，使用一系列实验，包括最低抑菌浓度、生长曲线和抑菌曲线，检测了（OAPO）有机酸对的抗菌活性。基于细胞壁和细胞膜的完整性和通透性、扫描电子显微镜和可溶性蛋白质含量评估了抗菌机制。使用小鼠皮肤伤口愈合模型验证了OAPO对的抗菌作用。结果表明，OAPO不仅改善了皮肤伤口愈合，还降低了皮肤伤口中的细菌载量。此外，OAPO治疗组中的炎症细胞和细胞因子数量减少。总之，本研究报道了一种能够抑制和的植物提取物，表明OAPO在不久的将来有预防和控制感染的潜在用途。
