Wu Yuying, Zhang Jun
Department of Surgical Oncology Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, China.
Front Oncol. 2023 Jan 13;12:1083570. doi: 10.3389/fonc.2022.1083570. eCollection 2022.
To search for the DEGs between stage MS NB and stage M NB and speculate the possible mechanism of spontaneous regression of stage MS NB.
The NB datasets GSE49710 and GSE45547 in the GEO database were selected to screen the DEGs between children with NB stage MS vs. stage M, < 18 months. GO enrichment and KEGG pathway analysis of DEGs was performed using DAVID. The intersecting genes among DEGs and RCD-related genes were selected, and their survival roles and functions were assessed. We then used the collected clinical samples to validate the expression of these genes at the protein level using IHC methods and further analysis to explore their role.
BIRC5, SLCO4A1, POPDC3, and HK2 were found to be downregulated in stage MS NB and related to apoptosis. BIRC5 and HK2 also participate in autophagy. The TF gene is upregulated in stage MS NB and related to ferroptosis. The above five genes are closely related to the survival of children with NB. And the expression levels of all five genes at the protein level were verified by IHC to be consistent with the results of the preliminary screening described above.
BIRC5, SLCO4A1, POPDC3, HK2 and TF are expected to become new important indicators to predict the prognosis of NB and can be used as the basis for further explored the benign prognosis and spontaneous regression mechanism of stage MS NB.
寻找MS期神经母细胞瘤（NB）与M期NB之间的差异表达基因（DEGs），并推测MS期NB自发消退的可能机制。
选择GEO数据库中的NB数据集GSE49710和GSE45547，以筛选18个月以下NB患儿MS期与M期之间的DEGs。使用DAVID对DEGs进行基因本体（GO）富集分析和京都基因与基因组百科全书（KEGG）通路分析。选择DEGs与细胞程序性死亡（RCD）相关基因之间的交集基因，并评估其生存作用和功能。然后，我们使用收集的临床样本，通过免疫组化（IHC）方法在蛋白质水平验证这些基因的表达，并进一步分析以探索其作用。
发现BIRC5、SLCO4A1、POPDC3和HK2在MS期NB中下调，且与细胞凋亡相关。BIRC5和HK2也参与自噬。TF基因在MS期NB中上调，且与铁死亡相关。上述五个基因与NB患儿的生存密切相关。通过IHC验证，这五个基因在蛋白质水平的表达与上述初步筛选结果一致。
BIRC5、SLCO4A1、POPDC3、HK2和TF有望成为预测NB预后的新的重要指标，并可作为进一步探索MS期NB良性预后和自发消退机制的依据。
