Fay M W, Poland J L, Breen T J
Department of Physiology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
Proc Soc Exp Biol Med. 1987 Nov;186(2):192-7. doi: 10.3181/00379727-186-42602.
The purpose of the present study was to compare dexamethasone-induced glycogen increases in normal EDL and SOL muscles with that in free muscle grafts. Glycogen in mature EDL and SOL grafts in the rat equalled control concentrations irrespective of whether the graft was a nerve-intact (NI), nerve-crushed (NC), reimplanted, or cross-transplanted graft. The grafts also possessed the glycogen-regulatory mechanisms to respond to the glucocorticoid dexamethasone (DEX), which increases muscle glycogen. The increase in glycogen induced by DEX in the EDL and SOL grafts resembled that of the EDL and SOL muscles, respectively, whether the grafted muscle was originally an EDL or SOL. DEX induced an approximate twofold increase in glycogen concentration in control muscles and nerve-intact SOL grafts, and a smaller but significant increase in all other free grafts. Nerve crushing prior to grafting resulted in no significant change in muscle weight, glycogen concentration, or DEX-induced glycogen increase in these grafts. The data suggest that skeletal muscle grafts are qualitatively similar to normal muscles in terms of metabolic responsiveness to hormones. Leaving the nerve intact during grafting quantitatively enhances the graft's hormonal sensitivity but the technique of nerve crushing prior to grafting has no such effect.
本研究的目的是比较地塞米松诱导正常趾长伸肌（EDL）和比目鱼肌（SOL）中糖原增加的情况与游离肌肉移植中糖原增加的情况。无论移植的肌肉是神经完整（NI）、神经挤压（NC）、再植入还是交叉移植，大鼠成熟的EDL和SOL移植肌肉中的糖原含量均与对照浓度相当。这些移植肌肉还具有糖原调节机制，能够对增加肌肉糖原的糖皮质激素地塞米松（DEX）作出反应。无论移植的肌肉原本是EDL还是SOL，DEX诱导的EDL和SOL移植肌肉中糖原的增加分别类似于EDL和SOL肌肉中糖原的增加。DEX使对照肌肉和神经完整的SOL移植肌肉中的糖原浓度增加了约两倍，而在所有其他游离移植肌肉中，糖原增加幅度较小但具有显著性。移植前进行神经挤压，这些移植肌肉的重量、糖原浓度或DEX诱导的糖原增加均无显著变化。数据表明，骨骼肌移植在对激素的代谢反应方面在质量上与正常肌肉相似。移植时保留神经在数量上会增强移植肌肉对激素的敏感性，但移植前进行神经挤压的技术则没有这种效果。
