Mong F S, Poland J L, Poland J W
Can J Physiol Pharmacol. 1982 Mar;60(3):387-91. doi: 10.1139/y82-056.
The metabolic integrity of skeletal muscle grafts must be restored, along with histological regeneration, if muscle transplants are to be clinically acceptable. The purpose of the present study was to contrast glycogen changes in muscle grafts after exercise or fasting with those in normal muscles. The extensor digitorum longus (EDL) and soleus (SOL) muscles were switched to each other's muscle bed in one leg of male Sprague-Dawley rats. Fifty days later, some of the rats were fasted for 48 h and sacrificed either immediately or after 4 h of refeeding. Other rats were run on a treadmill for 30 min ( 1 mi/h (0.447 m/s)) and sacrificed either immediately or after 4 h of resting. Muscle grafts and contralateral normal muscles of these experimental, as well as control animals (neither exercised nor fasted), were removed for determination of glycogen concentration. The results show that glycogen in muscle grafts, as in normal muscles, decreases with exercise or fasting. After 4 h of rest or refeeding, glycogen is restored in both grafts and normal muscles. It is concluded, therefore, that muscle grafts are metabolically active and that their pattern of utilization and restoration of glycogen in response to physiological events, such as exercise or fasting, is similar to that of normal muscle.U
如果肌肉移植要在临床上被接受，就必须恢复骨骼肌移植物的代谢完整性以及组织学再生。本研究的目的是对比运动或禁食后肌肉移植物与正常肌肉中糖原的变化。在雄性Sprague-Dawley大鼠的一条腿上，将趾长伸肌（EDL）和比目鱼肌（SOL）相互移植到对方的肌床。50天后，一些大鼠禁食48小时，然后立即处死，或在重新喂食4小时后处死。其他大鼠在跑步机上以1英里/小时（0.447米/秒）的速度跑30分钟，然后立即处死，或在休息4小时后处死。取出这些实验动物以及对照动物（既不运动也不禁食）的肌肉移植物和对侧正常肌肉，测定糖原浓度。结果表明，与正常肌肉一样，肌肉移植物中的糖原会随着运动或禁食而减少。在休息或重新喂食4小时后，移植物和正常肌肉中的糖原都会恢复。因此得出结论，肌肉移植物具有代谢活性，并且它们在响应运动或禁食等生理事件时糖原的利用和恢复模式与正常肌肉相似。
