Mori Nanase, Kawasaki Hideya, Nishida Erika, Kanemoto Yukimi, Miyaji Hirofumi, Umeda Junko, Kondoh Katsuyoshi
Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials and Bioengineering, Kansai University, Suita-shi, Osaka 564-8689 Japan.
Department of Periodontology and Endodontology, Faculty of Dental Medicine, Hokkaido University, Sapporo, Hokkaido 060-8586 Japan.
J Mater Sci. 2023;58(6):2801-2813. doi: 10.1007/s10853-023-08194-z. Epub 2023 Jan 25.
Rice husks are well known for their high silica content, and the RH-derived silica nanoparticles (RH NPs) are amorphous and biocompatible; therefore, they are suitable raw materials for biomedical applications. In this study, rose bengal-impregnated rice husk nanoparticles (RB-RH NPs) were prepared for their potential photosensitization and O generation as antimicrobial photodynamic inactivation. RB is a halogen-xanthene type's photosensitizer showing high singlet oxygen efficiency, and the superior photophysical properties are desirable for RB in the antimicrobial photodynamic inactivation of bacteria. To enhance the binding of anionic RB to RH NPs, we conducted cationization for the RH NPs using polyethyleneimine (PEI). The control of the RB adsorption state on cationic PEI-modified RH NPs was essential for RB RH-NP photosensitizers to obtain efficient O generation. Minimizing RB aggregation allowed highly efficient O production from RB-RH NPs at the molar ratio of RB with the PEI, X = 0.1. The RB-RH NPs have significant antimicrobial activity against compared to free RB after white light irradiation. The RB-RH NP-based antimicrobial photodynamic inactivation can be employed effectively in treating for dental applications.
The online version contains supplementary material available at 10.1007/s10853-023-08194-z.
稻壳以其高硅含量而闻名，从稻壳衍生的二氧化硅纳米颗粒（RH NPs）是无定形且具有生物相容性的；因此，它们是生物医学应用的合适原材料。在本研究中，制备了孟加拉玫瑰红浸渍的稻壳纳米颗粒（RB-RH NPs），以发挥其潜在的光敏化作用并产生单线态氧用于抗菌光动力失活。孟加拉玫瑰红是一种卤代呫吨型光敏剂，具有较高的单线态氧效率，其优异的光物理性质使其在细菌的抗菌光动力失活中具有优势。为了增强阴离子型孟加拉玫瑰红与稻壳纳米颗粒的结合，我们使用聚乙烯亚胺（PEI）对稻壳纳米颗粒进行了阳离子化处理。控制阳离子型PEI修饰的稻壳纳米颗粒上孟加拉玫瑰红的吸附状态对于RB-RH NP光敏剂获得高效的单线态氧生成至关重要。将孟加拉玫瑰红的聚集最小化可使RB-RH NPs在孟加拉玫瑰红与PEI的摩尔比X = 0.1时高效产生单线态氧。与游离的孟加拉玫瑰红相比，白光照射后RB-RH NPs对[具体对象未提及]具有显著的抗菌活性。基于RB-RH NPs的抗菌光动力失活可有效地用于牙科应用中治疗[具体疾病未提及]。
在线版本包含可在10.1007/s10853-023-08194-z获取的补充材料。
