Wang Haoyu, Xiao Pengli, Sang Shengbo, Chen Honglie, Dong Xiushan, Ge Yang, Guo Xing, Zhao Dong
Shanxi Key Laboratory of Micro Nano Sensors & Artificial Intelligence Perception, College of Information and Computer, Taiyuan University of Technology, Taiyuan 030024, China.
Shanxi Bethune Hospital, Taiyuan 030024, China.
ACS Omega. 2023 Jan 9;8(3):3423-3428. doi: 10.1021/acsomega.2c07338. eCollection 2023 Jan 24.
A factor closely associated with renal disease status in clinical diagnosis is abnormal human serum albumin (HSA) concentration levels in human body fluids urine, serum, etc. The surface stress biosensor was developed as a new type of biosensor to detect protein molecule concentration and has a wide range of clinical applications. However, further sensitivity improvement is required to achieve higher detection performance. Herein, MXene/PDMS/FeO/PDMS of the multilayer heterogeneous membrane biosensor (MHBios) based on the coupling of the magnetic field, electric field, and surface stress field was successfully developed to achieve high sensitivity HSA detection through magnetic sensitization. The modified antibody specifically binds to HSA at the AuNP layer, allowing the biosensor to convert the surface stress caused by PDMS film deformation into an electrical signal. When the biosensor was exposed to a uniform magnetic field, the conductive path of the conductive layer was reshaped further as the magnetic force amplified the deformation of the PDMS film, enhancing the conversion of biological signals to electrical signals. The results exhibited that the detection limit (LOD) of the MHBios was 78 ng/mL when HSA concentration was 0-50 μg/mL, which was markedly lower than the minimum diagnostic limit of microalbuminuria. Furthermore, the MHBios detected HSA in actual samples, confirming the potential for early disease screening.
在临床诊断中，与肾脏疾病状态密切相关的一个因素是人体体液（尿液、血清等）中人类血清白蛋白（HSA）浓度水平异常。表面应力生物传感器作为一种新型生物传感器被开发出来，用于检测蛋白质分子浓度，具有广泛的临床应用。然而，为了实现更高的检测性能，还需要进一步提高灵敏度。在此，基于磁场、电场和表面应力场耦合的多层异质膜生物传感器（MHBios）的MXene/PDMS/FeO/PDMS被成功开发出来，通过磁敏化实现对HSA的高灵敏度检测。修饰后的抗体在金纳米颗粒层特异性结合HSA，使生物传感器能够将由聚二甲基硅氧烷（PDMS）薄膜变形引起的表面应力转化为电信号。当生物传感器暴露在均匀磁场中时，随着磁力放大PDMS薄膜的变形，导电层的导电路径进一步重塑，增强了生物信号向电信号的转化。结果表明，当HSA浓度为0 - 50μg/mL时，MHBios的检测限（LOD）为78 ng/mL，明显低于微量白蛋白尿的最低诊断限。此外，MHBios在实际样品中检测到了HSA，证实了其在疾病早期筛查方面的潜力。
