Shanxi Key Laboratory of Micro Nano Sensors & Artificial Intelligence Perception, College of Information and Computer, Taiyuan University of Technology, Taiyuan, 030024, China.
Shanxi Bethune Hospital, Taiyuan, 030024, China.
Anal Biochem. 2023 Sep 15;677:115264. doi: 10.1016/j.ab.2023.115264. Epub 2023 Jul 27.
The conventional mechanical biosensor based on stress and electrical conversion can be an effective method to detect key human biomarkers for clinical diagnosis and early disease prevention. However, the applications of this type of biosensor are greatly limited due to their unsatisfactory sensitivity. In this work, a magnetic-sensitized (MS) mechanical biosensor based on multi-field coupling was developed for higher sensitivity, giving access to detect human serum albumin (HSA). Via introducing secondary magnetic antibodies labeled with magnetized FeO nanoparticles to the stress and electrical conversion element of the MS-biosensor, the multi-field coupling was realized based on stress, electricity, and magnetism. Under the action of the magnetic field, the magnetic force of the secondary magnetic antibody and the stress of antigen-antibody binding jointly drove and enhanced the deformation of the MS-biosensor, amplifying the electrical signal, and realizing magnetic sensitization. The HSA was detected by the MS-biosensor at a range of 0-80 μg/mL with a limit of detection (LOD) of 0.14 μg/mL, demonstrating the high performance of the MS-biosensor. Moreover, the MS-biosensor showed high selectivity, specificity, and stability, indicating that the magnetic sensitization strategy of the MS-biosensor was significant for the clinical application of mechanical biosensors.
基于应力和电转换的传统机械生物传感器可以成为检测关键人体生物标志物以用于临床诊断和早期疾病预防的有效方法。然而，由于其灵敏度不理想，这种类型的生物传感器的应用受到了极大的限制。在这项工作中，开发了一种基于多场耦合的磁敏（MS）机械生物传感器，以实现更高的灵敏度，从而能够检测人血清白蛋白（HSA）。通过将标记有磁化 FeO 纳米颗粒的二次磁性抗体引入到 MS 生物传感器的应力和电转换元件中，基于应力、电和磁实现了多场耦合。在磁场的作用下，二次磁性抗体的磁力和抗原-抗体结合的应力共同驱动并增强了 MS 生物传感器的变形，放大了电信号，实现了磁敏化。MS 生物传感器在 0-80μg/mL 的范围内检测到 HSA，检测限（LOD）为 0.14μg/mL，显示出 MS 生物传感器的高性能。此外，MS 生物传感器表现出高选择性、特异性和稳定性，表明 MS 生物传感器的磁敏化策略对机械生物传感器的临床应用具有重要意义。
