Yousefifard Mahmoud, Sarveazad Arash, Janzadeh Atousa, Behroozi Zahra, Nasirinezhad Farinaz
Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran.
Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran.
J Stem Cells Regen Med. 2022 Dec 31;18(2):53-63. doi: 10.46582/jsrm.1802010. eCollection 2022.
Few studies are conducted on the efficacy of human adipose-derived stem cells (ADSCs) in spinal cord injury (SCI) management and electrophysiological changes in the spinal cord. Therefore, the present study aimed to determine the effect of ADSCs on neuropathic pain, motor function recovery, and electrophysiology assessment. For the purpose of this study, adult male Wistar rats (weight: 140-160 gr, n = 42) were randomly allocated into five groups namely intact animals, sham-operated, SCI non-treated animals, vehicle-treated (culture media), and ADSCs treated groups. One week after clips compression SCI induction, about 1×10 cells were transplanted into the spinal cord. As well, both neuropathic pain (allodynia and hyperalgesia) and motor function were measured weekly. Cavity size, ADSCs survival, and electrophysiology assessments were measured at the end of the eighth week. The transplantation of ADSCs resulted in a significant improvement in the locomotion of SCI animals (p<0.0001), mechanical allodynia (p<0.0001), cold allodynia (p<0.0001), mechanical hyperalgesia (p<0.0001), and thermal hyperalgesia (p<0.0001). The cavity size was significantly smaller among the ADSCs-treated animals (p <0.0001). The single-unit recording showed that the transplantation of ADSCs decreased wide dynamic range (WDR) in neurons and it evoked potential in response to receiving signals from Aβ (p<0.0001) and Aδ (p=0.003) C-fiber (p<0.0001) neurons. Post-discharge recorded from WDR neurons decreased after the transplantation of ADSCs (p<0.0001) and wind up in the ADSCs-treated group was lower than that of the SCI group (p=0.003). Our results showed that the transplantation of ADSCs could significantly alleviate neuropathic pain, enhance motor function recovery, and improve electrophysiology findings after SCI.
很少有关于人脂肪来源干细胞（ADSCs）在脊髓损伤（SCI）治疗中的疗效以及脊髓电生理变化的研究。因此，本研究旨在确定ADSCs对神经性疼痛、运动功能恢复和电生理评估的影响。为了本研究的目的，将成年雄性Wistar大鼠（体重：140 - 160克，n = 42）随机分为五组，即完整动物组、假手术组、未治疗的SCI动物组、载体治疗组（培养基）和ADSCs治疗组。在夹闭压迫诱导SCI一周后，将约1×10个细胞移植到脊髓中。此外，每周测量神经性疼痛（痛觉过敏和痛觉超敏）和运动功能。在第八周结束时测量空洞大小、ADSCs存活情况和电生理评估。ADSCs移植导致SCI动物的运动能力（p<0.0001）、机械性痛觉过敏（p<0.0001）、冷痛觉过敏（p<0.0001）、机械性痛觉超敏（p<0.0001）和热痛觉超敏（p<0.0001）有显著改善。在ADSCs治疗的动物中，空洞大小显著更小（p <0.0001）。单单位记录显示，ADSCs移植减少了神经元的广动力范围（WDR），并且它在接收到来自Aβ（p<0.0001）、Aδ（p = 0.003）和C纤维（p<0.0001）神经元的信号时诱发电位。从WDR神经元记录的放电后活动在ADSCs移植后减少（p<0.0001），并且ADSCs治疗组的wind up低于SCI组（p = 0.003）。我们的结果表明，ADSCs移植可以显著减轻神经性疼痛，促进运动功能恢复，并改善SCI后的电生理结果。
