Nahar Saifun, Nakashima Yoshiki, Miyagi-Shiohira Chika, Kinjo Takao, Toyoda Zensei, Kobayashi Naoya, Saitoh Issei, Watanabe Masami, Noguchi Hirofumi, Fujita Jiro
Department of Infectious, Respiratory, and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa 903-0215, Japan.
Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa 903-0215, Japan.
World J Stem Cells. 2018 Nov 26;10(11):146-159. doi: 10.4252/wjsc.v10.i11.146.
Adipose-derived mesenchymal stem cells (ADSCs) are a treatment cell source for patients with chronic liver injury. ADSCs are characterized by being harvested from the patient's own subcutaneous adipose tissue, a high cell yield (., reduced immune rejection response), accumulation at a disease nidus, suppression of excessive immune response, production of various growth factors and cytokines, angiogenic effects, anti-apoptotic effects, and control of immune cells via cell-cell interaction. We previously showed that conditioned medium of ADSCs promoted hepatocyte proliferation and improved the liver function in a mouse model of acute liver failure. Furthermore, as found by many other groups, the administration of ADSCs improved liver tissue fibrosis in a mouse model of liver cirrhosis. A comprehensive protein expression analysis by liquid chromatography with tandem mass spectrometry showed that the various cytokines and chemokines produced by ADSCs promote the healing of liver disease. In this review, we examine the ability of expressed protein components of ADSCs to promote healing in cell therapy for liver disease. Previous studies demonstrated that ADSCs are a treatment cell source for patients with chronic liver injury. This review describes the various cytokines and chemokines produced by ADSCs that promote the healing of liver disease.
脂肪来源的间充质干细胞（ADSCs）是慢性肝损伤患者的一种治疗性细胞来源。ADSCs的特点是从患者自身皮下脂肪组织中获取，细胞产量高（即免疫排斥反应降低），可在病灶处聚集，抑制过度免疫反应，产生多种生长因子和细胞因子，具有血管生成作用、抗凋亡作用，并通过细胞间相互作用控制免疫细胞。我们之前表明，在急性肝衰竭小鼠模型中，ADSCs的条件培养基可促进肝细胞增殖并改善肝功能。此外，正如其他许多研究小组所发现的，在肝硬化小鼠模型中，ADSCs的给药可改善肝组织纤维化。通过液相色谱串联质谱法进行的全面蛋白质表达分析表明，ADSCs产生的各种细胞因子和趋化因子可促进肝病的愈合。在本综述中，我们研究了ADSCs表达的蛋白质成分在肝病细胞治疗中促进愈合的能力。先前的研究表明，ADSCs是慢性肝损伤患者的一种治疗性细胞来源。本综述描述了ADSCs产生的各种促进肝病愈合的细胞因子和趋化因子。
