印度尼西亚中爪哇省马格朗市普罗戈河亚洲红尾鲶（瓦朗谢讷，1840年）的最新细胞色素氧化酶亚基I分子检测
The newest COI molecular detection of Asian redtail catfish (Valenciennes, 1840) in Progo River, Magelang, Central Java, Indonesia.
作者信息
Yuanawati Desyiamililia, Farizky Hutama Satriana, Santanumurti Muhammad Browijoyo, Jamal Mamdoh T, Iqbal Sani Lalu M, Madduppa Hawis, Sari Putri Desi Wulan
机构信息
Program Study of Aquaculture, Faculty of Fisheries and Marine, Universitas Airlangga, Surabaya, Indonesia.
Department of Aquaculture, Faculty of Fisheries and Marine, Universitas Airlangga, Surabaya, Indonesia.
出版信息
J Adv Vet Anim Res. 2022 Nov 18;9(4):591-600. doi: 10.5455/javar.2022.i628. eCollection 2022 Dec.
OBJECTIVE
This study describes the newest deoxyribonucleic acid (DNA) barcoding Asian redtail catfish () in the Progo River, Magelang, Central Java, Indonesia.
MATERIALS AND METHODS
Ten fish were caught in the Progo River, Magelang, Central Java, Indonesia. The polymerase chain reaction was the molecular diagnosis to detect the sequences of DNA of Cytochrome Oxidase I compared to National Center for Biotechnology Information data (GenBank).
RESULTS
The results showed that the percent identity was not 100% with data from other locations (GenBank), including Indonesia. The closest percent identity was from Java Island (Accession ID: MK312566.1) with 97.6% similarity. The genetic mutation that happened might be due to environmental change (pollution) in the Progo River recently.
CONCLUSIONS
This study showed a genetic mutation in from Progo River may be caused by environmental change. Low pollution exposure levels may not be detrimental (lethal) to fish. However, it can affect fish fertility, which leads to population degradation (gene variation). Attention must be increased for fish survival in the future.
目的
本研究描述了印度尼西亚中爪哇省马格朗市普罗戈河中最新的亚洲红尾鲶（ ）的脱氧核糖核酸（DNA）条形码。
材料与方法
在印度尼西亚中爪哇省马格朗市的普罗戈河中捕获了10条鱼。聚合酶链反应是一种分子诊断方法，用于与美国国立生物技术信息中心的数据（基因库）比较检测细胞色素氧化酶I的DNA序列。
结果
结果表明，与其他地点（包括印度尼西亚）的数据（基因库）相比，同一性百分比并非100%。最接近的同一性百分比来自爪哇岛（登录号：MK312566.1），相似度为97.6%。最近普罗戈河中发生的基因突变可能是由于环境变化（污染）所致。
结论
本研究表明，普罗戈河中的 发生的基因突变可能是由环境变化引起的。低污染暴露水平可能不会对鱼类有害（致死）。然而，它会影响鱼类的繁殖力，从而导致种群退化（基因变异）。未来必须更加关注鱼类的生存。
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