Lin Qingfang, Gong Jiaxin, Zhang Zhiliang, Meng Zizi, Wang Jianyong, Wang Song, Sun Jing, Gu Xu, Jin Yuting, Wu Tong, Yan Nuo, Wang Yuxin, Kai Lei, Jiang Jihong, Qi Shilian
School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu, China.
Institute of Genetic and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Front Plant Sci. 2023 Jan 13;13:1088278. doi: 10.3389/fpls.2022.1088278. eCollection 2022.
Roots are the main organs through which plants absorb water and nutrients. As the key phytohormone involved in root growth, auxin functions in plant environmental responses by modulating auxin synthesis, distribution and polar transport. The gene can improve root architecture, and mutants have significantly shortened primary roots. However, the mechanism underlying the short roots of the mutant and the upstream signaling pathway and downstream genes regulated by are unclear. Here, we demonstrated that the gene could promote auxin accumulation in -overexpressing plants. By comparing the transcriptomic data of and wild-type roots, we found several upregulations of auxin-related genes, including , and , may play an important role in the gene-mediated auxin transport signaling pathway, ultimately leading to changes in auxin content and primary root length. Moreover, increased expression can regulate primary root growth and lateral root elongation under different concentration of nitrate conditions. Overall, constitutive expression of increased auxin contents and improved lateral root elongation, constituting a new method for improving the nitrogen utilization efficiency of plants.
根是植物吸收水分和养分的主要器官。作为参与根生长的关键植物激素，生长素通过调节生长素的合成、分布和极性运输在植物对环境的反应中发挥作用。该基因可改善根系结构，而该基因的突变体主根显著缩短。然而，该突变体根短的潜在机制以及该基因调控的上游信号通路和下游基因尚不清楚。在这里，我们证明该基因可促进过表达植株中生长素的积累。通过比较该基因过表达植株和野生型根的转录组数据，我们发现几个生长素相关基因的上调，包括[具体基因名称1]、[具体基因名称2]和[具体基因名称3]，可能在该基因介导的生长素运输信号通路中起重要作用，最终导致生长素含量和主根长度的变化。此外，在不同浓度硝酸盐条件下，该基因表达的增加可调节主根生长和侧根伸长。总体而言，该基因的组成型表达增加了生长素含量并改善了侧根伸长，构成了一种提高植物氮利用效率的新方法。
